Differential regulation of glucose transporter activity and expression in red and white skeletal muscle

Jeanne M. Richardson, Thomas W. Balon, Judith L. Treadway, Jeffrey E. Pessin

Research output: Contribution to journalArticle

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Abstract

Insulin-stimulated glucose transport activity and GLUT4 glucose transporter protein expression in rat soleus, red-enriched, and white-enriched skeletal muscle were examined in streptozotocin (STZ)-induced insulin-deficient diabetes. Six days of STZ-diabetes resulted in a nearly complete inhibition of insulin-stimulated glucose transport activity in perfused soleus, red, and white muscle which recovered following insulin therapy. A specific decrease in the GLUT4 glucose transporter protein was observed in soleus (3-fold) and red (2-fold) muscle which also recovered to control values with insulin therapy. Similarly, cardiac muscle displayed a marked STZ-induced decrease in GLUT4 protein that was normalized by insulin therapy. White muscle displayed a small but statistically significant decrease in GLUT4 protein (23%), but this could not account for the marked inhibition of insulin-stimulated glucose transport activity observed in this tissue. In addition, GLUT4 mRNA was found to decrease in red muscle (2-fold) with no significant alteration in white muscle. The effect of STZ-induced diabetes was time-dependent with maximal inhibition of insulin-stimulated glucose transport activity at 24 h in both red and white skeletal muscle and half-maximal inhibition at approximately 8 h. In contrast, GLUT4 protein in red and white muscle remained unchanged until 4 and 7 days following STZ treatment, respectively. These data demonstrate that red skeletal muscle displays a more rapid hormonal/metabolic-dependent regulation of GLUT4 glucose transporter protein and mRNA expression than white skeletal muscle. In addition, the inhibition of insulin-stimulated glucose transport activity in both red and white muscle precedes the decrease in GLUT4 protein and mRNA levels. Thus, STZ treatment initially results in a rapid uncoupling of the insulin-mediated signaling of glucose transport activity which is independent of GLUT4 protein and mRNA levels.

Original languageEnglish (US)
Pages (from-to)12690-12694
Number of pages5
JournalJournal of Biological Chemistry
Volume266
Issue number19
StatePublished - 1991
Externally publishedYes

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Facilitative Glucose Transport Proteins
Muscle
Skeletal Muscle
Glucose Transporter Type 4
Insulin
Streptozocin
Muscles
Glucose
Medical problems
Messenger RNA
Experimental Diabetes Mellitus
Proteins
Myocardium
Therapeutics
Rats

ASJC Scopus subject areas

  • Biochemistry

Cite this

Differential regulation of glucose transporter activity and expression in red and white skeletal muscle. / Richardson, Jeanne M.; Balon, Thomas W.; Treadway, Judith L.; Pessin, Jeffrey E.

In: Journal of Biological Chemistry, Vol. 266, No. 19, 1991, p. 12690-12694.

Research output: Contribution to journalArticle

Richardson, Jeanne M. ; Balon, Thomas W. ; Treadway, Judith L. ; Pessin, Jeffrey E. / Differential regulation of glucose transporter activity and expression in red and white skeletal muscle. In: Journal of Biological Chemistry. 1991 ; Vol. 266, No. 19. pp. 12690-12694.
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