Differential profiles of immune mediators and in vitro HIV infectivity between endocervical and vaginal secretions from women with Chlamydia trachomatis infection

A pilot study

Rhoda Sperling, Thomas A. Kraus, Jian Ding, Alina Veretennikova, Elizabeth Lorde-Rollins, Tricia Singh, Yungtai Lo, Alison J. Quayle, Theresa L. Chang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Chlamydia trachomatis infection is one of the most prevalent bacterial STIs in the USA and worldwide, and women with C. trachomatis infection are at increased risk of acquiring HIV. Because immune activation at the genital mucosa facilitates HIV/SIV infection, C. trachomatis-mediated cytokine induction may contribute to increased HIV transmission in asymptomatic women. To begin to elucidate the mechanisms, we longitudinally analyzed profiles of innate immune factors and HIV infectivity in genital secretions from anatomically specific sites in asymptomatic women during C. trachomatis infection and post-antibiotic treatment. We found higher levels of cytokines and chemokines in endocervical secretions than vaginal secretions. Compared with the convalescent state, G-CSF, IL-1α, and RANTES were elevated in endocervical secretions, IFN-γ and TNF-α were elevated in vaginal secretions, and IFNγ, IL-1β, and MIP1-α were elevated in cervicolavage fluid (CVL), before adjustment of multiple comparisons. Elevated endocervical levels of IP-10 and MCP-1 were associated with the use of hormonal contraception in infected women after successful treatment, suggesting the role of hormonal contraception in inflammation independent of STIs. Importantly, soluble factors found in endocervical secretions during infection enhanced HIV infectivity while no difference in HIV infectivity was found with vaginal secretions or CVL during infection or at convalescence. Taken together, the profiles of immune mediators and in vitro HIV infectivity indicate that the endocervical and vaginal mucosa are immunologically distinct. Our results underscore the importance of considering anatomical site and local sampling methodology when measuring mucosal responses, particularly in the presence of C. trachomatis infection.

Original languageEnglish (US)
Pages (from-to)80-87
Number of pages8
JournalJournal of Reproductive Immunology
Volume99
Issue number1-2
DOIs
StatePublished - Sep 2013

Fingerprint

Chlamydia Infections
Chlamydia trachomatis
HIV
Sexually Transmitted Diseases
Contraception
Interleukin-1
HIV Infections
Mucous Membrane
Cytokines
Chemokine CCL5
Macrophage Colony-Stimulating Factor
Immunologic Factors
Granulocyte Colony-Stimulating Factor
Chemokines
In Vitro Techniques
Anti-Bacterial Agents
Inflammation
Therapeutics
Infection

Keywords

  • Chlamydia trachomatis
  • HIV infectivity in vitro.
  • HIV transmission
  • Immune mediators
  • Lower genital tract

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Differential profiles of immune mediators and in vitro HIV infectivity between endocervical and vaginal secretions from women with Chlamydia trachomatis infection : A pilot study. / Sperling, Rhoda; Kraus, Thomas A.; Ding, Jian; Veretennikova, Alina; Lorde-Rollins, Elizabeth; Singh, Tricia; Lo, Yungtai; Quayle, Alison J.; Chang, Theresa L.

In: Journal of Reproductive Immunology, Vol. 99, No. 1-2, 09.2013, p. 80-87.

Research output: Contribution to journalArticle

Sperling, Rhoda ; Kraus, Thomas A. ; Ding, Jian ; Veretennikova, Alina ; Lorde-Rollins, Elizabeth ; Singh, Tricia ; Lo, Yungtai ; Quayle, Alison J. ; Chang, Theresa L. / Differential profiles of immune mediators and in vitro HIV infectivity between endocervical and vaginal secretions from women with Chlamydia trachomatis infection : A pilot study. In: Journal of Reproductive Immunology. 2013 ; Vol. 99, No. 1-2. pp. 80-87.
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abstract = "Chlamydia trachomatis infection is one of the most prevalent bacterial STIs in the USA and worldwide, and women with C. trachomatis infection are at increased risk of acquiring HIV. Because immune activation at the genital mucosa facilitates HIV/SIV infection, C. trachomatis-mediated cytokine induction may contribute to increased HIV transmission in asymptomatic women. To begin to elucidate the mechanisms, we longitudinally analyzed profiles of innate immune factors and HIV infectivity in genital secretions from anatomically specific sites in asymptomatic women during C. trachomatis infection and post-antibiotic treatment. We found higher levels of cytokines and chemokines in endocervical secretions than vaginal secretions. Compared with the convalescent state, G-CSF, IL-1α, and RANTES were elevated in endocervical secretions, IFN-γ and TNF-α were elevated in vaginal secretions, and IFNγ, IL-1β, and MIP1-α were elevated in cervicolavage fluid (CVL), before adjustment of multiple comparisons. Elevated endocervical levels of IP-10 and MCP-1 were associated with the use of hormonal contraception in infected women after successful treatment, suggesting the role of hormonal contraception in inflammation independent of STIs. Importantly, soluble factors found in endocervical secretions during infection enhanced HIV infectivity while no difference in HIV infectivity was found with vaginal secretions or CVL during infection or at convalescence. Taken together, the profiles of immune mediators and in vitro HIV infectivity indicate that the endocervical and vaginal mucosa are immunologically distinct. Our results underscore the importance of considering anatomical site and local sampling methodology when measuring mucosal responses, particularly in the presence of C. trachomatis infection.",
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