Differential inflammatory response to acrylonitrile in rat primary astrocytes and microglia

Samuel W. Caito, Yingchun Yu, Michael Aschner

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Acrylonitrile (ACN) is extensively used in the production of plastics, resins, nitriles and other commercial products. Chronic low dose exposures to ACN cause glial cell tumors in rats, primarily microglial in origin. Recently it has been determined that astrocytes and microglia respond to ACN-induced oxidative stress differently, which may influence cell-specific activation of inflammatory and carcinogenic pathways. This study was conducted to compare the inflammatory responses of astrocytes and microglia following ACN treatment in vitro to further characterize differential sensitivities and adaptive responses in these cell types. Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and p53 levels were measured along with levels of 12 different cytokines and chemokines in primary rat microglia and astrocytes. Additionally levels of cytochrome P450 2E1 (CYP2E1) were measured to evaluate the cells' ability to metabolize ACN. Results indicate that while both cells upregulate p53 and NF-κB, the cytokines and chemokines produced differ between the cell types. Astrocytes, but not microglia, upregulated CYP2E1 in response to ACN, which may be due to the astrocytes accumulating more ACN than the microglia. Altogether our data implicate the inflammatory response as an important event in ACN-induced neurotoxicity.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalNeuroToxicology
Volume42
DOIs
StatePublished - 2014

Fingerprint

Acrylonitrile
Microglia
Astrocytes
Rats
Cytochrome P-450 CYP2E1
Chemokines
Nitrile resins
Cytokines
Nitriles
Oxidative stress
Glioma
Plastics
Tumors
Oxidative Stress
B-Lymphocytes
Up-Regulation
Chemical activation
Cells
Light

Keywords

  • Acrylonitrile
  • Astrocytes
  • Inflammation
  • Microglia
  • NF-κB
  • P53

ASJC Scopus subject areas

  • Neuroscience(all)
  • Toxicology
  • Medicine(all)

Cite this

Differential inflammatory response to acrylonitrile in rat primary astrocytes and microglia. / Caito, Samuel W.; Yu, Yingchun; Aschner, Michael.

In: NeuroToxicology, Vol. 42, 2014, p. 1-7.

Research output: Contribution to journalArticle

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