Differential gene expression between visceral and subcutaneous fat depots

G. Atzmon, X. M. Yang, R. Muzumdar, X. H. Ma, I. Gabriely, Nir Barzilai

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

Abdominal obesity has been linked to the development of insulin resistance and Type 2 diabetes mellitus (DM2). By surgical removal of visceral fat (VF) in a variety of rodent models, we prevented insulin resistance and glucose intolerance, establishing a cause-effect relationship between VF and the metabolic syndrome. To characterize the biological differences between visceral and peripheral fat depots, we obtained perirenal visceral (VF) and subcutaneous (SC) fat from 5 young rats. We extracted mRNA from the fat tissue and performed gene array hybridization using Affymetrix technology with a platform containing 9 000 genes. Out of the 1 660 genes that were expressed in fat tissue, 297 (17.9%) genes show a two-fold or higher difference in their expression between the two tissues. We present the 20 genes whose expression is higher in VF fat (by 3-7 fold) and the 20 genes whose expression is higher in SC fat (by 3-150 fold), many of which are predominantly involved in glucose homeostasis, insulin action, and lipid metabolism. We confirmed the findings of gene array expression and quantified the changes in expression in VF of genes involved in insulin resistance (PPARγ leptin) and its syndrome (angiotensinogen and plasminogen activating inhibitor-1, PAI-1) by real-time PCR (qRT-PCR) technology. Finally, we demonstrated increased expression of resistin in VF by around 12-fold and adiponectin by around 4-fold, peptides that were not part of the gene expression platform. These results indicate that visceral fat and subcutaneous fat are biologically distinct.

Original languageEnglish (US)
Pages (from-to)622-628
Number of pages7
JournalHormone and Metabolic Research
Volume34
Issue number11-12
DOIs
StatePublished - Nov 2002

Fingerprint

Intra-Abdominal Fat
Subcutaneous Fat
Gene expression
Fats
Gene Expression
Genes
Insulin Resistance
Insulin
Technology
Resistin
Angiotensinogen
Peroxisome Proliferator-Activated Receptors
Tissue
Glucose Intolerance
Abdominal Obesity
Plasminogen
Adiponectin
Leptin
Lipid Metabolism
Type 2 Diabetes Mellitus

Keywords

  • Adiponectin (ACRP30)
  • Angiotensinogen
  • Leptin
  • Plasminogen Activating Inhibitor-1 (PAI-1)
  • PPARγ
  • Resistin
  • Subcutaneous Fat
  • Visceral Fat

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Differential gene expression between visceral and subcutaneous fat depots. / Atzmon, G.; Yang, X. M.; Muzumdar, R.; Ma, X. H.; Gabriely, I.; Barzilai, Nir.

In: Hormone and Metabolic Research, Vol. 34, No. 11-12, 11.2002, p. 622-628.

Research output: Contribution to journalArticle

Atzmon, G. ; Yang, X. M. ; Muzumdar, R. ; Ma, X. H. ; Gabriely, I. ; Barzilai, Nir. / Differential gene expression between visceral and subcutaneous fat depots. In: Hormone and Metabolic Research. 2002 ; Vol. 34, No. 11-12. pp. 622-628.
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