Differential expression of three gap junction proteins in developing and mature brain tissues

R. Dermietzel, O. Traub, T. K. Hwang, E. Beyer, Michael V. L. Bennett, David C. Spray, K. Willecke

Research output: Contribution to journalArticle

428 Citations (Scopus)

Abstract

By using antibodies directed against gap junction proteins of liver (connexins 26 and 32) and heart (connexin 43), we have localized immunoreactivity to specific cell types in frozen sections of adult rodent brains. Connexin 32 reactivity was found in oligodendrocytes and also in a few neurons, whereas reactivity to connexins 26 and 43 was localized to leptomeningeal cells, ependymal cells, and pineal gland. Immunoreactivity with antibodies to connexin 43 also occurred in astrocytes. Furthermore, during embryonic and postnatal maturation of brain tissues, gap junction proteins were differentially expressed. Connexins 43 and 26 predominated in the neuroepithelium of embryonic brains, whereas connexin 32 was virtually absent. Between 3 and 6 weeks after birth, connexin 26 largely disappeared from immature brain; this time course corresponded to the increased expression of connexin 32. Expression of connexin 43 remained high throughout embryonic and postnatal development. These findings demonstrate that gap junction expression in the brain is diverse, with specific cell types expressing different connexins; this cell-specific distribution may imply differences in the function of these intercellular channels in different loci and developmental stages.

Original languageEnglish (US)
Pages (from-to)10148-10152
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number24
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Connexin 43
Connexins
Brain
Pineal Gland
Antibodies
Gap Junctions
Oligodendroglia
Frozen Sections
Astrocytes
Embryonic Development
Rodentia
Parturition
Neurons
connexin 32
Connexin 26
Liver

Keywords

  • connexin 26
  • connexin 32
  • connexin 43
  • Electronic synapses

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Differential expression of three gap junction proteins in developing and mature brain tissues. / Dermietzel, R.; Traub, O.; Hwang, T. K.; Beyer, E.; Bennett, Michael V. L.; Spray, David C.; Willecke, K.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, No. 24, 1989, p. 10148-10152.

Research output: Contribution to journalArticle

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