Differential expression of circulating microRNAs according to severity of colorectal neoplasia

Gloria Y F Ho, Hwa Jin Jung, Robert E. Schoen, Tao Wang, Juan Lin, Zev Williams, Joel L. Weissfeld, Jung Y. Park, Olivier Loudig, Yousin Suh

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

There is a need to develop a colorectal cancer (CRC) screening test that is noninvasive, cost effective, and sensitive enough to detect preneoplastic lesions. This case-control study examined the feasibility of using circulating extracellular microRNAs (miRNAs) to differentiate a spectrum of colorectal neoplasia of various severity and hence for early detection of colorectal neoplasia. Archived serum samples of 10 normal controls and 31 cases, including 10 with nonadvanced adenoma, 10 with advanced adenoma, and 11 with CRC, were profiled for circulating miRNAs using next-generation sequencing. Multiple linear regression, adjusting for age, gender, and smoking status, compared controls and the 3 case groups for levels of 175 miRNAs that met stringent criteria for miRNA sequencing analysis. Of the 175 miRNAs, 106 miRNAs were downregulated according to severity of neoplasia and showed a relative decrease in the expression from controls to nonadvanced adenoma to advanced adenoma to CRC (P<inf>trend</inf> < 0.05). Pairwise group comparisons showed that 39 and 80 miRNAs were differentially expressed in the advanced adenoma and CRC groups compared with the controls, respectively. Differences in miRNA levels between the nonadvanced adenoma group and controls were modest. Our study found that expression of many miRNAs in serum was inversely correlated with the severity of colorectal neoplasia, and differential miRNA profiles were apparent in preneoplastic cases with advanced lesions, suggesting circulating miRNAs could serve as potential biomarkers for CRC screening.

Original languageEnglish (US)
Pages (from-to)225-232
Number of pages8
JournalTranslational Research
Volume166
Issue number3
DOIs
StatePublished - Sep 1 2015

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MicroRNAs
Adenoma
Neoplasms
Colorectal Neoplasms
Early Detection of Cancer
Screening
Biomarkers
Serum
Linear regression
Case-Control Studies
Linear Models
Down-Regulation
Smoking
Costs and Cost Analysis
Control Groups

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, medical
  • Public Health, Environmental and Occupational Health

Cite this

Differential expression of circulating microRNAs according to severity of colorectal neoplasia. / Ho, Gloria Y F; Jung, Hwa Jin; Schoen, Robert E.; Wang, Tao; Lin, Juan; Williams, Zev; Weissfeld, Joel L.; Park, Jung Y.; Loudig, Olivier; Suh, Yousin.

In: Translational Research, Vol. 166, No. 3, 01.09.2015, p. 225-232.

Research output: Contribution to journalArticle

Ho, Gloria Y F ; Jung, Hwa Jin ; Schoen, Robert E. ; Wang, Tao ; Lin, Juan ; Williams, Zev ; Weissfeld, Joel L. ; Park, Jung Y. ; Loudig, Olivier ; Suh, Yousin. / Differential expression of circulating microRNAs according to severity of colorectal neoplasia. In: Translational Research. 2015 ; Vol. 166, No. 3. pp. 225-232.
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