Differential expression of adenosine deaminase isozymes in acute leukemia

H. Ratech, F. Martiniuk, W. Z. Borer, H. Rappaport

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Total adenosine deaminase (ADA) and purine nucleoside phosphorylase (PNP) activities were measured in cell samples from 13 cases of de novo acute leukemia and from three cases of chronic myeloid leukemia in blast crisis (CMLBC). These cases could be separated into lymphoid and nonlymphoid types on the basis of enzyme activity, with two misclassifications. However, PNP activity added little or no discriminatory information. Analysis for expression of the various molecular weight (mol wt) ADA isozymes, ADA1 (40 Kd) and ADA2 (110 Kd), revealed that ADA2 was expressed exclusively in nonlymphoid cells whereas ADA1 was found in both lymphoid and nonlymphoid cell types. Identification of ADA2 divided these leukemia cases into lymphoid and nonlymphoid types with no misclassification (P = .0002; Fisher's exact test). Acute nonlymphoblastic leukemia (ANLL) with a monocytic component tended to have a greater percentage of ADA2 than ANLL without a monocytic component. These studies suggest that ADA2 may be a novel biochemical marker for an immature nonlymphoid cell.

Original languageEnglish (US)
Pages (from-to)1627-1632
Number of pages6
JournalBlood
Volume72
Issue number5
StatePublished - 1988
Externally publishedYes

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Purine-Nucleoside Phosphorylase
Adenosine Deaminase
Isoenzymes
Leukemia
Acute Myeloid Leukemia
Enzyme activity
Blast Crisis
Molecular weight
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Biomarkers
Molecular Weight
Lymphocytes
Enzymes

ASJC Scopus subject areas

  • Hematology

Cite this

Ratech, H., Martiniuk, F., Borer, W. Z., & Rappaport, H. (1988). Differential expression of adenosine deaminase isozymes in acute leukemia. Blood, 72(5), 1627-1632.

Differential expression of adenosine deaminase isozymes in acute leukemia. / Ratech, H.; Martiniuk, F.; Borer, W. Z.; Rappaport, H.

In: Blood, Vol. 72, No. 5, 1988, p. 1627-1632.

Research output: Contribution to journalArticle

Ratech, H, Martiniuk, F, Borer, WZ & Rappaport, H 1988, 'Differential expression of adenosine deaminase isozymes in acute leukemia', Blood, vol. 72, no. 5, pp. 1627-1632.
Ratech H, Martiniuk F, Borer WZ, Rappaport H. Differential expression of adenosine deaminase isozymes in acute leukemia. Blood. 1988;72(5):1627-1632.
Ratech, H. ; Martiniuk, F. ; Borer, W. Z. ; Rappaport, H. / Differential expression of adenosine deaminase isozymes in acute leukemia. In: Blood. 1988 ; Vol. 72, No. 5. pp. 1627-1632.
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