Differential endotoxin sensitivity of lymphocytes and macrophages from mice with an X-linked defect in B cell maturation

David L. Rosenstreich, S. N. Vogel, A. Jacques, L. M. Wahl, I. Scher, S. E. Mergenhagen

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The in vitro sensitivity of B lymphocytes and macrophages derived from (CBA/N x DBA/2N)F 1 male mice, which carry an X-linked recessive gene that produces defective B cell maturation, was compared to phenotypically normal F 1 female mice. B lymphocytes of F 1 males exhibit an abnormal mitogenic response to LPS in serum-free culture conditions, which is partially reversed in the presence of serum. In contrast, both resident and thioglycollate-induced peritoneal macrophages of F 1 male mice respond normally to LPS. In response to LPS in vitro, F 1 male macrophages produce the monokine, lymphocyte-activating factor (LAF) and release prostaglandins. Furthermore, F 1 male macrophages are sensitive to the lethal effects of LPS. Therefore, the defective CBA/N gene appears to be expressed only in B lymphocytes and not in macrophages. Since F 1 male mice are normally sensitive to the lethal and adjuvant effects of LPS in vivo, these findings suggest that a mature B lymphocyte population is not required for these effects and support the role of the macrophage in the mediation of LPS-induced lethality and adjuvanticity.

Original languageEnglish (US)
Pages (from-to)685-690
Number of pages6
JournalJournal of Immunology
Volume121
Issue number2
StatePublished - 1978
Externally publishedYes

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Endotoxins
B-Lymphocytes
Macrophages
Lymphocytes
Monokines
Thioglycolates
Recessive Genes
X-Linked Genes
Peritoneal Macrophages
Serum
Interleukin-1
Prostaglandins
Population
Genes
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology

Cite this

Differential endotoxin sensitivity of lymphocytes and macrophages from mice with an X-linked defect in B cell maturation. / Rosenstreich, David L.; Vogel, S. N.; Jacques, A.; Wahl, L. M.; Scher, I.; Mergenhagen, S. E.

In: Journal of Immunology, Vol. 121, No. 2, 1978, p. 685-690.

Research output: Contribution to journalArticle

Rosenstreich, David L. ; Vogel, S. N. ; Jacques, A. ; Wahl, L. M. ; Scher, I. ; Mergenhagen, S. E. / Differential endotoxin sensitivity of lymphocytes and macrophages from mice with an X-linked defect in B cell maturation. In: Journal of Immunology. 1978 ; Vol. 121, No. 2. pp. 685-690.
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