Differential effects of interleukin-4 in peptide induced autoimmunity

Bisram Deocharan; Paola Marambio; Morris Edelman; Chaim Putterman

doi:10.1016/S1521-6616(03)00096-2

Differential effects of interleukin-4 in peptide induced autoimmunity

Bisram Deocharan, Paola Marambio, Morris Edelman, Chaim Putterman

Medicine

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

BALB/c mice immunized with multimeric DWEYSVWLSN develop IgG1 anti-DNA antibodies and glomerular immunoglobulin deposits, leading us to investigate the role of IL-4 in this model of antigen induced lupus. Splenocytes from DWEYSVWLSN immunized mice secreted IL-4 but not γ-interferon. Following peptide immunization, IgG1 anti-peptide and anti-DNA antibodies were significantly higher in IL-4 wild type mice, while IgM and IgG3 anti-DNA levels were significantly higher in IL-4 knockout mice. Titers of IgG anti-laminin and anti-histone, but not anti-Sm/RNP and anti-cardiolipin antibodies, were significantly higher in the IL-4 wild type group. Glomerular immunoglobulin deposition was substantially decreased in IL-4 knockout mice. We conclude that while IL-4 does not materially affect the generation of some autoantibody responses associated with peptide induced autoimmunity, IL-4 deficiency inhibits kidney immunoglobulin deposition. The effect of IL-4 on humoral autoimmunity in lupus is complex, and is dependent on genetic background, the antigenic trigger and stage of disease.

Original language	English (US)
Pages (from-to)	80-88
Number of pages	9
Journal	Clinical Immunology
Volume	108
Issue number	2
DOIs	https://doi.org/10.1016/S1521-6616(03)00096-2
State	Published - Aug 1 2003

Keywords

Anti-DNA antibodies
Autoantibodies
Cytokines
Glomerulonephritis
Interleukin-4
Peptide mimetope
SLE

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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title = "Differential effects of interleukin-4 in peptide induced autoimmunity",

abstract = "BALB/c mice immunized with multimeric DWEYSVWLSN develop IgG1 anti-DNA antibodies and glomerular immunoglobulin deposits, leading us to investigate the role of IL-4 in this model of antigen induced lupus. Splenocytes from DWEYSVWLSN immunized mice secreted IL-4 but not γ-interferon. Following peptide immunization, IgG1 anti-peptide and anti-DNA antibodies were significantly higher in IL-4 wild type mice, while IgM and IgG3 anti-DNA levels were significantly higher in IL-4 knockout mice. Titers of IgG anti-laminin and anti-histone, but not anti-Sm/RNP and anti-cardiolipin antibodies, were significantly higher in the IL-4 wild type group. Glomerular immunoglobulin deposition was substantially decreased in IL-4 knockout mice. We conclude that while IL-4 does not materially affect the generation of some autoantibody responses associated with peptide induced autoimmunity, IL-4 deficiency inhibits kidney immunoglobulin deposition. The effect of IL-4 on humoral autoimmunity in lupus is complex, and is dependent on genetic background, the antigenic trigger and stage of disease.",

keywords = "Anti-DNA antibodies, Autoantibodies, Cytokines, Glomerulonephritis, Interleukin-4, Peptide mimetope, SLE",

author = "Bisram Deocharan and Paola Marambio and Morris Edelman and Chaim Putterman",

note = "Funding Information: This work was supported in part by a National Institute of Health grant RO1 AR48692-01, and an Arthritis Investigator grant from the Arthritis Foundation (to C.P.). We wish to thank Dr. Betty Diamond for many helpful discussions and suggestions.",

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AU - Edelman, Morris

AU - Putterman, Chaim

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Differential effects of interleukin-4 in peptide induced autoimmunity

Abstract

Keywords

ASJC Scopus subject areas

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