Differential effects of interleukin-4 in peptide induced autoimmunity

Bisram Deocharan, Paola Marambio, Morris Edelman, Chaim Putterman

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

BALB/c mice immunized with multimeric DWEYSVWLSN develop IgG1 anti-DNA antibodies and glomerular immunoglobulin deposits, leading us to investigate the role of IL-4 in this model of antigen induced lupus. Splenocytes from DWEYSVWLSN immunized mice secreted IL-4 but not γ-interferon. Following peptide immunization, IgG1 anti-peptide and anti-DNA antibodies were significantly higher in IL-4 wild type mice, while IgM and IgG3 anti-DNA levels were significantly higher in IL-4 knockout mice. Titers of IgG anti-laminin and anti-histone, but not anti-Sm/RNP and anti-cardiolipin antibodies, were significantly higher in the IL-4 wild type group. Glomerular immunoglobulin deposition was substantially decreased in IL-4 knockout mice. We conclude that while IL-4 does not materially affect the generation of some autoantibody responses associated with peptide induced autoimmunity, IL-4 deficiency inhibits kidney immunoglobulin deposition. The effect of IL-4 on humoral autoimmunity in lupus is complex, and is dependent on genetic background, the antigenic trigger and stage of disease.

Original languageEnglish (US)
Pages (from-to)80-88
Number of pages9
JournalClinical Immunology
Volume108
Issue number2
DOIs
StatePublished - Aug 1 2003

Fingerprint

Autoimmunity
Interleukin-4
Peptides
Immunoglobulins
Immunoglobulin G
Antinuclear Antibodies
Knockout Mice
Cardiolipins
Laminin
Autoantibodies
Histones
Interferons
Anti-Idiotypic Antibodies
Immunization
Kidney
Antigens
DNA

Keywords

  • Anti-DNA antibodies
  • Autoantibodies
  • Cytokines
  • Glomerulonephritis
  • Interleukin-4
  • Peptide mimetope
  • SLE

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Differential effects of interleukin-4 in peptide induced autoimmunity. / Deocharan, Bisram; Marambio, Paola; Edelman, Morris; Putterman, Chaim.

In: Clinical Immunology, Vol. 108, No. 2, 01.08.2003, p. 80-88.

Research output: Contribution to journalArticle

Deocharan, Bisram ; Marambio, Paola ; Edelman, Morris ; Putterman, Chaim. / Differential effects of interleukin-4 in peptide induced autoimmunity. In: Clinical Immunology. 2003 ; Vol. 108, No. 2. pp. 80-88.
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