Differential effects of γ-interferon on collagen and fibronectin gene expression

M. J. Czaja, F. R. Weiner, M. Eghbali, M. A. Giambrone, M. Eghbali, M. A. Zern

Research output: Contribution to journalArticle

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Abstract

The interferons are a group of endogenous proteins that exhibit a variety of biological functions in addition to their ability to induce resistance to viruses. In order to evaluate the anti-fibrogenic actions of interferon, we have delineated the level of regulation responsible for γ-interferon-induced changes in collagen and fibronectin gene expression in cultured fibroblasts. Confluent human skin fibroblasts were exposed to 500 anti-viral units/ml of γ-interferon. RNA was then extracted from the cells, and steady-state mRNA levels were determined by Northern and dot blot hybridization studies. Cells exposed to interferon had type I procollagen mRNA levels that were 23% of control and type III procollagen mRNA levels only 7% of control. The interferon-treated cells also had β-actin mRNA levels that were decreased to 51% that of untreated cells but had fibronectin steady-state mRNA levels that were 560% of control levels. Nuclear run-on assays revealed that interferon did not affect the transcriptional rates of types I and III collagen or β-actin, but it did increase the transcriptional rate of fibronectin to 670% of control levels. These findings demonstrate that γ-interferon causes a marked decrease in types I and III procollagen mRNA levels in vitro by a posttranscriptional mechanism while inducing fibronectin expression at a transcriptional level.

Original languageEnglish (US)
Pages (from-to)13348-13351
Number of pages4
JournalJournal of Biological Chemistry
Volume262
Issue number27
StatePublished - 1987

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Fibronectins
Gene expression
Interferons
Collagen
Gene Expression
Messenger RNA
Collagen Type III
Level control
Fibroblasts
Collagen Type I
Actins
Procollagen
Interferon Type I
Northern Blotting
Viruses
Assays
Skin
RNA
Cells
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Czaja, M. J., Weiner, F. R., Eghbali, M., Giambrone, M. A., Eghbali, M., & Zern, M. A. (1987). Differential effects of γ-interferon on collagen and fibronectin gene expression. Journal of Biological Chemistry, 262(27), 13348-13351.

Differential effects of γ-interferon on collagen and fibronectin gene expression. / Czaja, M. J.; Weiner, F. R.; Eghbali, M.; Giambrone, M. A.; Eghbali, M.; Zern, M. A.

In: Journal of Biological Chemistry, Vol. 262, No. 27, 1987, p. 13348-13351.

Research output: Contribution to journalArticle

Czaja, MJ, Weiner, FR, Eghbali, M, Giambrone, MA, Eghbali, M & Zern, MA 1987, 'Differential effects of γ-interferon on collagen and fibronectin gene expression', Journal of Biological Chemistry, vol. 262, no. 27, pp. 13348-13351.
Czaja MJ, Weiner FR, Eghbali M, Giambrone MA, Eghbali M, Zern MA. Differential effects of γ-interferon on collagen and fibronectin gene expression. Journal of Biological Chemistry. 1987;262(27):13348-13351.
Czaja, M. J. ; Weiner, F. R. ; Eghbali, M. ; Giambrone, M. A. ; Eghbali, M. ; Zern, M. A. / Differential effects of γ-interferon on collagen and fibronectin gene expression. In: Journal of Biological Chemistry. 1987 ; Vol. 262, No. 27. pp. 13348-13351.
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