Differential effect of selective block of α2-adrenopeceptors on plasma levels of tumour necrosis factor-α, interleukin-6 and corticosterone induced by bacterial lipopolysaccharide in mice

G. Hasko, I. J. Elenkov, Vladimir Kvetan, E. S. Vizi

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Abstract

The effect of selective block of α2-adrenoreceptors on plasma levels of tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6) and corticosterone induced by bacterial lipopolysaccharide (LPS) was investigated in mice using ELISA and RIA. it was found that the LPS-induced TNF-α response was significantly blunted in mice pretreated with CH-38083, a novel and highly selective α2-adrenoreceptor antagonist (the α21 ratio is >2000). In contrast, LPS-induced increases in both corticosterone and IL-6 plasma levels were further increased by CH-38083. Since it has recently been shown that the selective block of α2-adrenoreceptors located on noradrenergic axon terminals resulted in an increase in the release of noradrenaline (NA), both in the central and peripheral nervous systems, and, in our experiments, that propranolol prevented the effect of α2-adrenoreceptor blockade on TNF-α plasma levels induced by LPS, it seems likely that the excessive stimulation by NA of β-adrenoreceptors located on cytokine-secreting immune cells is responsible for this action. Since it is generally accepted that increased production of TNF-α is involved in the pathogenesis of inflammation and endotoxin shock on the one hand, and corticosterone and even IL-6 are known to possess anti-inflammatory properties on the other hand, it is suggested that the selective block of α2-adrenoreceptors might be beneficial in the treatment of inflammation and/or endotoxin shock.

Original languageEnglish (US)
Pages (from-to)457-462
Number of pages6
JournalJournal of Endocrinology
Volume144
Issue number3
StatePublished - 1995
Externally publishedYes

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Corticosterone
Lipopolysaccharides
Interleukin-6
Tumor Necrosis Factor-alpha
Endotoxins
Shock
Norepinephrine
Inflammation
Presynaptic Terminals
Peripheral Nervous System
Propranolol
Anti-Inflammatory Agents
Central Nervous System
Enzyme-Linked Immunosorbent Assay
Cytokines
7,8-(methylenedioxy)-14-hydroxyberbane
Therapeutics

ASJC Scopus subject areas

  • Endocrinology

Cite this

Differential effect of selective block of α2-adrenopeceptors on plasma levels of tumour necrosis factor-α, interleukin-6 and corticosterone induced by bacterial lipopolysaccharide in mice. / Hasko, G.; Elenkov, I. J.; Kvetan, Vladimir; Vizi, E. S.

In: Journal of Endocrinology, Vol. 144, No. 3, 1995, p. 457-462.

Research output: Contribution to journalArticle

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