Differential distribution of signal-transducing G-proteins in retina

Rajnikant P. Lad, Cyrena Simons, Peter Gierschik, Graeme Milligan, Charles Woodaard, Mark Griffo, Paul Goldsmith, Richard Ornberg, Charles R. Gerfen, Allen Spiegel

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


We used specific antibodies in immunoblot studies of membrane fractions derived from bovine retina, and in immunohistochemical studies of sections of rat retina to determine the distribution of two guanine nucleotide binding proteins Go (a G-protein of unknown function discovered in the brain) and transducin, in retina. Both Go and transducin were readily detected in membranes derived from whole retina, and in crude rod outer segment membranes. Purification of rod outer segment membranes by sucrose density gradient centrifugation resulted in enrichment in transducin and depletion of Go immunoreactivity. Transducin-α immunoreactivity was localized to photoreceptor inner and outer segments and the outer nuclear layer. In contrast, Go-α immunoreactivity was localized in the inner and outer plexiform layers and ganglion cell layer. The results indicate that Go unlike transducin, is not associated with rod outer segment membranes and is therefore unlikely to function in phototransduction. Go is, however, relatively abundant in neural layers of retina where it may be involved in signal transduction.

Original languageEnglish (US)
Pages (from-to)237-246
Number of pages10
JournalBrain research
Issue number1-2
StatePublished - Oct 13 1987
Externally publishedYes


  • Guanine nucleotide-binding protein
  • Signal transduction

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology


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