Differential developmental regulation of rat liver canalicular membrane transporters Bsep and Mrp2

Gitit Tomer, Meenakshisundaram Ananthanarayanan, Alexander Weymann, Natarajan Balasubramanian, Frederick J. Suchy

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Bile formation depends on the active secretion of bile salts and other biliary constituents by specific transporters. Recently two major transporters that contribute to bile formation, the bile salt export pump (Bsep) and multidrug resistance protein-2 (Mrp2), have been cloned. The goal of the present study was to define the expression of Bsep and Mrp2 during rat liver development, mRNA expression as assessed by Northern blot and RT-PCR was higher for Mrp2 (40% of adult) at 21 d fetal age relative to Bsep (<20% of adult). The levels of Mrp2 mRNA increased to ∼50% of adult at 1 d of life and then rapidly increased to adult levels by 1-3 wk. Nuclear run-on assays for Bsep and Mrp2 showed minimal transcription during fetal life with an increase in transcription in the postnatal period. A different pattern of expression was observed for both Mrp2 and Bsep proteins. During fetal life, there was low expression of Mrp2 and Bsep proteins (<20% of adult) with a gradual increase neonatally reaching adult levels at 4 wk. Thus, we noted a temporal delay between the maximal expression of the mRNA (1-3 wk) and protein (4 wk) for Bsep and Mrp2. These results show that 1) expression (of mRNA and protein) of canalicular transporters is developmentally regulated by both transcriptional and posttranscriptional mechanisms and 2) Mrp2 and Bsep gene expression (mRNA) are differentially regulated.

Original languageEnglish (US)
Pages (from-to)288-294
Number of pages7
JournalPediatric Research
Volume53
Issue number2
DOIs
StatePublished - Feb 1 2003
Externally publishedYes

Fingerprint

Membrane Transport Proteins
Bile Acids and Salts
Liver
Messenger RNA
Bile
Proteins
P-glycoprotein 2
Northern Blotting
Gestational Age
Gene Expression
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Differential developmental regulation of rat liver canalicular membrane transporters Bsep and Mrp2. / Tomer, Gitit; Ananthanarayanan, Meenakshisundaram; Weymann, Alexander; Balasubramanian, Natarajan; Suchy, Frederick J.

In: Pediatric Research, Vol. 53, No. 2, 01.02.2003, p. 288-294.

Research output: Contribution to journalArticle

Tomer, Gitit ; Ananthanarayanan, Meenakshisundaram ; Weymann, Alexander ; Balasubramanian, Natarajan ; Suchy, Frederick J. / Differential developmental regulation of rat liver canalicular membrane transporters Bsep and Mrp2. In: Pediatric Research. 2003 ; Vol. 53, No. 2. pp. 288-294.
@article{1210db45a2cb4a9190fab1ab00d84ea1,
title = "Differential developmental regulation of rat liver canalicular membrane transporters Bsep and Mrp2",
abstract = "Bile formation depends on the active secretion of bile salts and other biliary constituents by specific transporters. Recently two major transporters that contribute to bile formation, the bile salt export pump (Bsep) and multidrug resistance protein-2 (Mrp2), have been cloned. The goal of the present study was to define the expression of Bsep and Mrp2 during rat liver development, mRNA expression as assessed by Northern blot and RT-PCR was higher for Mrp2 (40{\%} of adult) at 21 d fetal age relative to Bsep (<20{\%} of adult). The levels of Mrp2 mRNA increased to ∼50{\%} of adult at 1 d of life and then rapidly increased to adult levels by 1-3 wk. Nuclear run-on assays for Bsep and Mrp2 showed minimal transcription during fetal life with an increase in transcription in the postnatal period. A different pattern of expression was observed for both Mrp2 and Bsep proteins. During fetal life, there was low expression of Mrp2 and Bsep proteins (<20{\%} of adult) with a gradual increase neonatally reaching adult levels at 4 wk. Thus, we noted a temporal delay between the maximal expression of the mRNA (1-3 wk) and protein (4 wk) for Bsep and Mrp2. These results show that 1) expression (of mRNA and protein) of canalicular transporters is developmentally regulated by both transcriptional and posttranscriptional mechanisms and 2) Mrp2 and Bsep gene expression (mRNA) are differentially regulated.",
author = "Gitit Tomer and Meenakshisundaram Ananthanarayanan and Alexander Weymann and Natarajan Balasubramanian and Suchy, {Frederick J.}",
year = "2003",
month = "2",
day = "1",
doi = "10.1203/01.PDR.0000047509.54253.01",
language = "English (US)",
volume = "53",
pages = "288--294",
journal = "Pediatric Research",
issn = "0031-3998",
publisher = "Lippincott Williams and Wilkins",
number = "2",

}

TY - JOUR

T1 - Differential developmental regulation of rat liver canalicular membrane transporters Bsep and Mrp2

AU - Tomer, Gitit

AU - Ananthanarayanan, Meenakshisundaram

AU - Weymann, Alexander

AU - Balasubramanian, Natarajan

AU - Suchy, Frederick J.

PY - 2003/2/1

Y1 - 2003/2/1

N2 - Bile formation depends on the active secretion of bile salts and other biliary constituents by specific transporters. Recently two major transporters that contribute to bile formation, the bile salt export pump (Bsep) and multidrug resistance protein-2 (Mrp2), have been cloned. The goal of the present study was to define the expression of Bsep and Mrp2 during rat liver development, mRNA expression as assessed by Northern blot and RT-PCR was higher for Mrp2 (40% of adult) at 21 d fetal age relative to Bsep (<20% of adult). The levels of Mrp2 mRNA increased to ∼50% of adult at 1 d of life and then rapidly increased to adult levels by 1-3 wk. Nuclear run-on assays for Bsep and Mrp2 showed minimal transcription during fetal life with an increase in transcription in the postnatal period. A different pattern of expression was observed for both Mrp2 and Bsep proteins. During fetal life, there was low expression of Mrp2 and Bsep proteins (<20% of adult) with a gradual increase neonatally reaching adult levels at 4 wk. Thus, we noted a temporal delay between the maximal expression of the mRNA (1-3 wk) and protein (4 wk) for Bsep and Mrp2. These results show that 1) expression (of mRNA and protein) of canalicular transporters is developmentally regulated by both transcriptional and posttranscriptional mechanisms and 2) Mrp2 and Bsep gene expression (mRNA) are differentially regulated.

AB - Bile formation depends on the active secretion of bile salts and other biliary constituents by specific transporters. Recently two major transporters that contribute to bile formation, the bile salt export pump (Bsep) and multidrug resistance protein-2 (Mrp2), have been cloned. The goal of the present study was to define the expression of Bsep and Mrp2 during rat liver development, mRNA expression as assessed by Northern blot and RT-PCR was higher for Mrp2 (40% of adult) at 21 d fetal age relative to Bsep (<20% of adult). The levels of Mrp2 mRNA increased to ∼50% of adult at 1 d of life and then rapidly increased to adult levels by 1-3 wk. Nuclear run-on assays for Bsep and Mrp2 showed minimal transcription during fetal life with an increase in transcription in the postnatal period. A different pattern of expression was observed for both Mrp2 and Bsep proteins. During fetal life, there was low expression of Mrp2 and Bsep proteins (<20% of adult) with a gradual increase neonatally reaching adult levels at 4 wk. Thus, we noted a temporal delay between the maximal expression of the mRNA (1-3 wk) and protein (4 wk) for Bsep and Mrp2. These results show that 1) expression (of mRNA and protein) of canalicular transporters is developmentally regulated by both transcriptional and posttranscriptional mechanisms and 2) Mrp2 and Bsep gene expression (mRNA) are differentially regulated.

UR - http://www.scopus.com/inward/record.url?scp=0037313267&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037313267&partnerID=8YFLogxK

U2 - 10.1203/01.PDR.0000047509.54253.01

DO - 10.1203/01.PDR.0000047509.54253.01

M3 - Article

VL - 53

SP - 288

EP - 294

JO - Pediatric Research

JF - Pediatric Research

SN - 0031-3998

IS - 2

ER -