Differences in nonsense-mediated mRNA decay activity in mammalian cell lines revealed by a fluorescence reporter

A. P. Pereverzev; M. E. Matlashov; D. B. Staroverov; K. A. Lukyanov; N. G. Gurskaya

doi:10.1134/S1068162015050118

Differences in nonsense-mediated mRNA decay activity in mammalian cell lines revealed by a fluorescence reporter

A. P. Pereverzev, M. E. Matlashov, D. B. Staroverov, K. A. Lukyanov, N. G. Gurskaya

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The activity of nonsense-mediated mRNA decay (NMD) cascade in several mammalian cell lines was investigated. The analysis was conducted with the help of the recently developed genetically encoded fluorescent sensor [Pereverzev et al.]. This fluorescent NMD-reporter allows estimating the NMD activity in single live cells from the ratio of signals of the green and red fluorescent proteins. The following cell lines were analyzed: mouse colon carcinoma CT26, mouse Lewis lung carcinoma LLC, human T-cell leukemia Jurkat, and spontaneously immortalized human keratinocytes HaCaT. These cell lines demonstrated very different NMD activities. The NMD activity in the CT26 cells was low, while in the LLC cells - high (8.5-fold higher than in CT26). The Jurkat and HaCaT cells demonstrated high heterogeneity and included two subpopulations of cells with low and high NMD activities. In addition, high NMD activity was demonstrated for the primary culture of mouse embryonic hippocampal neurons.

Original language	English (US)
Pages (from-to)	525-528
Number of pages	4
Journal	Russian Journal of Bioorganic Chemistry
Volume	41
Issue number	5
DOIs	https://doi.org/10.1134/S1068162015050118
State	Published - Sep 29 2015
Externally published	Yes

Keywords

NMD
cell culture
fluorescent protein
genetically encoded sensor

ASJC Scopus subject areas

Biochemistry
Organic Chemistry

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10.1134/S1068162015050118

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title = "Differences in nonsense-mediated mRNA decay activity in mammalian cell lines revealed by a fluorescence reporter",

abstract = "The activity of nonsense-mediated mRNA decay (NMD) cascade in several mammalian cell lines was investigated. The analysis was conducted with the help of the recently developed genetically encoded fluorescent sensor [Pereverzev et al.]. This fluorescent NMD-reporter allows estimating the NMD activity in single live cells from the ratio of signals of the green and red fluorescent proteins. The following cell lines were analyzed: mouse colon carcinoma CT26, mouse Lewis lung carcinoma LLC, human T-cell leukemia Jurkat, and spontaneously immortalized human keratinocytes HaCaT. These cell lines demonstrated very different NMD activities. The NMD activity in the CT26 cells was low, while in the LLC cells - high (8.5-fold higher than in CT26). The Jurkat and HaCaT cells demonstrated high heterogeneity and included two subpopulations of cells with low and high NMD activities. In addition, high NMD activity was demonstrated for the primary culture of mouse embryonic hippocampal neurons.",

keywords = "NMD, cell culture, fluorescent protein, genetically encoded sensor",

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AU - Pereverzev, A. P.

AU - Matlashov, M. E.

AU - Staroverov, D. B.

AU - Lukyanov, K. A.

AU - Gurskaya, N. G.

PY - 2015/9/29

Y1 - 2015/9/29

N2 - The activity of nonsense-mediated mRNA decay (NMD) cascade in several mammalian cell lines was investigated. The analysis was conducted with the help of the recently developed genetically encoded fluorescent sensor [Pereverzev et al.]. This fluorescent NMD-reporter allows estimating the NMD activity in single live cells from the ratio of signals of the green and red fluorescent proteins. The following cell lines were analyzed: mouse colon carcinoma CT26, mouse Lewis lung carcinoma LLC, human T-cell leukemia Jurkat, and spontaneously immortalized human keratinocytes HaCaT. These cell lines demonstrated very different NMD activities. The NMD activity in the CT26 cells was low, while in the LLC cells - high (8.5-fold higher than in CT26). The Jurkat and HaCaT cells demonstrated high heterogeneity and included two subpopulations of cells with low and high NMD activities. In addition, high NMD activity was demonstrated for the primary culture of mouse embryonic hippocampal neurons.

AB - The activity of nonsense-mediated mRNA decay (NMD) cascade in several mammalian cell lines was investigated. The analysis was conducted with the help of the recently developed genetically encoded fluorescent sensor [Pereverzev et al.]. This fluorescent NMD-reporter allows estimating the NMD activity in single live cells from the ratio of signals of the green and red fluorescent proteins. The following cell lines were analyzed: mouse colon carcinoma CT26, mouse Lewis lung carcinoma LLC, human T-cell leukemia Jurkat, and spontaneously immortalized human keratinocytes HaCaT. These cell lines demonstrated very different NMD activities. The NMD activity in the CT26 cells was low, while in the LLC cells - high (8.5-fold higher than in CT26). The Jurkat and HaCaT cells demonstrated high heterogeneity and included two subpopulations of cells with low and high NMD activities. In addition, high NMD activity was demonstrated for the primary culture of mouse embryonic hippocampal neurons.

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KW - cell culture

KW - fluorescent protein

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Differences in nonsense-mediated mRNA decay activity in mammalian cell lines revealed by a fluorescence reporter

Abstract

Keywords

ASJC Scopus subject areas

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