Differences in β‐adrenergic receptor density and adenylate cyclase activity between normal and leukaemic leukocytes

ELISABETH PAIETTA, JOSEF D. SCHWARZMEIER

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Abstract. An identical class of high‐affinity binding sites for the 125I‐labelled β‐adrenergic antagonist hydroxybenzylpindolol, was identified on intact human normal and leukaemic peripheral blood leukocytes. On normal unfractionated lymphocytes, polymorphonuclear leukocytes, and monocytes, receptor density did not differ significantly (1200–1400 receptors per cell; P > 0.3), but it was higher on B‐ than on T‐lymphocytes (P < 005). In leukaemia, monocytic blast cells expressed highest receptor numbers, whereas very low receptor density was seen on the pathologic B‐cells from chronic lymphocytic leukaemia. Among normal leukocytes, adenylate cyclase activation by hormones (isoproterenol, prostaglandin E1, histamine) and sodium fluoride was strongest in plasma membranes from monocytes, but very weak in polymorphonuclear leukocytes either due to uncoupling of hormone receptors from adenylate cyclase or to low catalytic activity. In T‐cells, enzyme activity was significantly lower than in B‐cells. Loss of adenylate cyclase sensitivity to hormones and fluoride occurred in leukaemic cells from chronic and acute lymphocytic leukaemia.

Original languageEnglish (US)
Pages (from-to)339-346
Number of pages8
JournalEuropean Journal of Clinical Investigation
Volume13
Issue number4
DOIs
StatePublished - Aug 1983
Externally publishedYes

Keywords

  • adenylate cyclase
  • catecholamines
  • leukaemic leukocytes
  • normal leukocytes
  • β‐adrenergic receptorsyy

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

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