Dietary n-3 Fatty Acids Increase Spleen Size and Postendotoxin Circulating TNF in Mice; Role of Macrophages, Macrophage Precursors, and Colony-Stimulating Factor-1

Willem L. Blok, Marella F.T.R. De Bruijn, Pieter J.M. Leenen, Wijnand M.C. Eling, Nico Van Rooijen, E. Richard Stanley, Wim A. Buurman, Jos W.M. Van Der Meer

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

In experimental studies in mice, dietary supplementation with n-3 fatty acids (FA) alleviates inflammation and increases resistance to infection. Nevertheless, TNF production capacity was found to be increased in n-3 FA-fed mice. We previously found increased relative spleen weights in n-3 FA-fed mice. In this study, the nature of this increased spleen size was further investigated. Spleen cellularity was increased significantly in mice fed n-3 FA (fish oil 15% w/w), compared with controls fed corn oil (15%) or normal lab chow (p < 0.05). Experiments with T cell-deficient nude mice and experiments using macrophage depletion through liposomal dichloromethylene-biphosphonate revealed that the increase in spleen cellularity is T cell independent and largely due to macrophage accumulation in the spleen. Accumulation of marginal zone and red pulp macrophages was histologically and immunohistochemically confirmed. n-3 FA induced peripheral blood monocytosis and an aspecific increase in bone marrow cellularity. Postendotoxin circulating TNF concentrations were increased significantly in n-3 FA-fed mice compared with controls. Splenectomy did not abolish this increase in circulating TNF. However, after macrophage depletion through liposomal dichloromethylene-biphosphonate, circulating TNF was not detectable after endotoxin challenge. Circulating concentrations of CSF-1 did not differ between the various experimental groups. It is suggested that the cellular changes observed relate to increased constitutive production of TNF.

Original languageEnglish (US)
Pages (from-to)5569-5573
Number of pages5
JournalJournal of Immunology
Volume157
Issue number12
StatePublished - Dec 15 1996
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Dietary n-3 Fatty Acids Increase Spleen Size and Postendotoxin Circulating TNF in Mice; Role of Macrophages, Macrophage Precursors, and Colony-Stimulating Factor-1'. Together they form a unique fingerprint.

  • Cite this

    Blok, W. L., De Bruijn, M. F. T. R., Leenen, P. J. M., Eling, W. M. C., Van Rooijen, N., Stanley, E. R., Buurman, W. A., & Van Der Meer, J. W. M. (1996). Dietary n-3 Fatty Acids Increase Spleen Size and Postendotoxin Circulating TNF in Mice; Role of Macrophages, Macrophage Precursors, and Colony-Stimulating Factor-1. Journal of Immunology, 157(12), 5569-5573.