Diego interacts with Prickle and Strabismus/Van Gogh to localize planar cell polarity complexes

Gishnu Das, Andreas Jenny, Thomas J. Klein, Suzanne Eaton, Marek Mlodzik

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

Planar cell polarity (PCP) in the Drosophila eye is established by the distinct fate specifications of photoreceptors R3 and R4, and is regulated by the Frizzled (Fz)/PCP signaling pathway. Before the PCP proteins become asymmetrically localized to opposite poles of the cell in response to Fz/PCP signaling, they are uniformly apically colocalized. Little is known about how the apical localization is maintained. We provide evidence that the PCP protein Diego (Dgo) promotes the maintenance of apical localization of Flamingo (Fmi), an atypical Cadherin-family member, which itself is required for the apical localization of the other PCP factors. This function of Dgo is redundant with Prickle (Pk) and Strabismus (Stbm), and only appreciable in double mutant tissue. We show that the initial membrane association of Dgo depends on Fz, and that Dgo physically interacts with Stbm and Pk through its Ankyrin repeats, providing evidence for a PCP multiprotein complex. These interactions suggest a positive feedback loop initiated by Fz that results in the apical maintenance of other PCP factors through Fmi.

Original languageEnglish (US)
Pages (from-to)4467-4476
Number of pages10
JournalDevelopment
Volume131
Issue number18
DOIs
StatePublished - Sep 2004
Externally publishedYes

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Cell Polarity
Strabismus
Maintenance
Ankyrin Repeat
Multiprotein Complexes
Cadherins
Drosophila
Proteins
Membranes

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Diego interacts with Prickle and Strabismus/Van Gogh to localize planar cell polarity complexes. / Das, Gishnu; Jenny, Andreas; Klein, Thomas J.; Eaton, Suzanne; Mlodzik, Marek.

In: Development, Vol. 131, No. 18, 09.2004, p. 4467-4476.

Research output: Contribution to journalArticle

Das, Gishnu ; Jenny, Andreas ; Klein, Thomas J. ; Eaton, Suzanne ; Mlodzik, Marek. / Diego interacts with Prickle and Strabismus/Van Gogh to localize planar cell polarity complexes. In: Development. 2004 ; Vol. 131, No. 18. pp. 4467-4476.
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