Diastolic properties, myocardial water content, and histologic condition of the rat left ventricle

Effect of varied osmolarity of a coronary perfusate

Y. M. Carter, C. X. Jia, P. F. Soto, J. P. Starr, D. G. Rabkin, Daphne T. Hsu, P. E. Fisher, H. M. Spotnitz

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: Although myocardial edema is known to impair diastolic filling of the left ventricle, the interrelation of edema, histologic condition, and function has not been quantitated sufficiently for extrapolation to studies of multifactorial influences on diastolic properties. Methods: Accordingly, ACI rat hearts arrested at 4°C underwent coronary artery perfusion with a cardioplegia solution that was either unaltered (288 mOsm/L, P288 group, n = 6), diluted (144 mOsm/L, P144 group, n = 6), or concentrated (380 mOsm/L, P380 group, n = 6). Postmortem left ventricular pressure-volume curves and myocardial water content were measured. Myocardial samples were fixed in varying dilutions of glutaraldehyde. After dehydration and paraffin embedding, edema was graded subjectively (0 to 5), and myocardial interstitial spaces were determined by use of a semiquantitative method. Results: Mean normalized left ventricular filling volume at 20 mm Hg filling pressure in the P144 group, 189 ± 16 μl (SEM), was reduced versus both the P288 (278 ± 26 μl) and the P380 (332 ± 18 μl) groups (p < 0.05, ANOVA). Mean myocardial water content in the P144 group, 80.7% ± 1%, was increased versus the P380 (76.7% ± 0.4%, p < 0.05) but not versus the P288 group (78.4% ± 0.8%). In hearts preserved with 2.5% glutaraldehyde, mean edema grade and interstitial space in the P144 group (4.0 ± 0.3) were increased versus the P380 (1.8 ± 0.3, p < 0.05) but not the P288 group (2.7 ± 0.5). Derived linear regressions relate water content to filling volume and histologic condition. Conclusions: Coronary perfusate osmolarity is thus associated with predictable changes in myocardial water content, left ventricular filling volume, and edema. These correlations allow definition of new hypotheses for the study of cardiac allograft rejection in patients and experimental animals.

Original languageEnglish (US)
Pages (from-to)140
Number of pages1
JournalJournal of Heart and Lung Transplantation
Volume17
Issue number2
StatePublished - 1998
Externally publishedYes

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Osmolar Concentration
Heart Ventricles
Edema
Water
Glutaral
Inbred ACI Rats
Paraffin Embedding
Induced Heart Arrest
Ventricular Pressure
Dehydration
Allografts
Linear Models
Coronary Vessels
Analysis of Variance
Perfusion
Pressure

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery
  • Transplantation

Cite this

Diastolic properties, myocardial water content, and histologic condition of the rat left ventricle : Effect of varied osmolarity of a coronary perfusate. / Carter, Y. M.; Jia, C. X.; Soto, P. F.; Starr, J. P.; Rabkin, D. G.; Hsu, Daphne T.; Fisher, P. E.; Spotnitz, H. M.

In: Journal of Heart and Lung Transplantation, Vol. 17, No. 2, 1998, p. 140.

Research output: Contribution to journalArticle

Carter, Y. M. ; Jia, C. X. ; Soto, P. F. ; Starr, J. P. ; Rabkin, D. G. ; Hsu, Daphne T. ; Fisher, P. E. ; Spotnitz, H. M. / Diastolic properties, myocardial water content, and histologic condition of the rat left ventricle : Effect of varied osmolarity of a coronary perfusate. In: Journal of Heart and Lung Transplantation. 1998 ; Vol. 17, No. 2. pp. 140.
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abstract = "Background: Although myocardial edema is known to impair diastolic filling of the left ventricle, the interrelation of edema, histologic condition, and function has not been quantitated sufficiently for extrapolation to studies of multifactorial influences on diastolic properties. Methods: Accordingly, ACI rat hearts arrested at 4°C underwent coronary artery perfusion with a cardioplegia solution that was either unaltered (288 mOsm/L, P288 group, n = 6), diluted (144 mOsm/L, P144 group, n = 6), or concentrated (380 mOsm/L, P380 group, n = 6). Postmortem left ventricular pressure-volume curves and myocardial water content were measured. Myocardial samples were fixed in varying dilutions of glutaraldehyde. After dehydration and paraffin embedding, edema was graded subjectively (0 to 5), and myocardial interstitial spaces were determined by use of a semiquantitative method. Results: Mean normalized left ventricular filling volume at 20 mm Hg filling pressure in the P144 group, 189 ± 16 μl (SEM), was reduced versus both the P288 (278 ± 26 μl) and the P380 (332 ± 18 μl) groups (p < 0.05, ANOVA). Mean myocardial water content in the P144 group, 80.7{\%} ± 1{\%}, was increased versus the P380 (76.7{\%} ± 0.4{\%}, p < 0.05) but not versus the P288 group (78.4{\%} ± 0.8{\%}). In hearts preserved with 2.5{\%} glutaraldehyde, mean edema grade and interstitial space in the P144 group (4.0 ± 0.3) were increased versus the P380 (1.8 ± 0.3, p < 0.05) but not the P288 group (2.7 ± 0.5). Derived linear regressions relate water content to filling volume and histologic condition. Conclusions: Coronary perfusate osmolarity is thus associated with predictable changes in myocardial water content, left ventricular filling volume, and edema. These correlations allow definition of new hypotheses for the study of cardiac allograft rejection in patients and experimental animals.",
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T1 - Diastolic properties, myocardial water content, and histologic condition of the rat left ventricle

T2 - Effect of varied osmolarity of a coronary perfusate

AU - Carter, Y. M.

AU - Jia, C. X.

AU - Soto, P. F.

AU - Starr, J. P.

AU - Rabkin, D. G.

AU - Hsu, Daphne T.

AU - Fisher, P. E.

AU - Spotnitz, H. M.

PY - 1998

Y1 - 1998

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AB - Background: Although myocardial edema is known to impair diastolic filling of the left ventricle, the interrelation of edema, histologic condition, and function has not been quantitated sufficiently for extrapolation to studies of multifactorial influences on diastolic properties. Methods: Accordingly, ACI rat hearts arrested at 4°C underwent coronary artery perfusion with a cardioplegia solution that was either unaltered (288 mOsm/L, P288 group, n = 6), diluted (144 mOsm/L, P144 group, n = 6), or concentrated (380 mOsm/L, P380 group, n = 6). Postmortem left ventricular pressure-volume curves and myocardial water content were measured. Myocardial samples were fixed in varying dilutions of glutaraldehyde. After dehydration and paraffin embedding, edema was graded subjectively (0 to 5), and myocardial interstitial spaces were determined by use of a semiquantitative method. Results: Mean normalized left ventricular filling volume at 20 mm Hg filling pressure in the P144 group, 189 ± 16 μl (SEM), was reduced versus both the P288 (278 ± 26 μl) and the P380 (332 ± 18 μl) groups (p < 0.05, ANOVA). Mean myocardial water content in the P144 group, 80.7% ± 1%, was increased versus the P380 (76.7% ± 0.4%, p < 0.05) but not versus the P288 group (78.4% ± 0.8%). In hearts preserved with 2.5% glutaraldehyde, mean edema grade and interstitial space in the P144 group (4.0 ± 0.3) were increased versus the P380 (1.8 ± 0.3, p < 0.05) but not the P288 group (2.7 ± 0.5). Derived linear regressions relate water content to filling volume and histologic condition. Conclusions: Coronary perfusate osmolarity is thus associated with predictable changes in myocardial water content, left ventricular filling volume, and edema. These correlations allow definition of new hypotheses for the study of cardiac allograft rejection in patients and experimental animals.

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