Diacylglycerol is required for the formation of COPI vesicles in the Golgi-to-ER transport pathway

Inés Fernández-Ulibarri, Montserrat Vilella, Francisco Lazaro-Dieguez, Elisabet Sarri, Susana E. Martínez, Nuria Jiménez, Enrique Claro, Isabel Mérida, Koert N J Burger, Gustavo Egea

Research output: Contribution to journalArticle

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Abstract

Diacylglycerol is necessary for trans-Golgi network (TGN) to cell surface transport, but its functional relevance in the early secretory pathway is unclear. Although depletion of diacylglycerol did not affect ER-to-Golgi transport, it led to a redistribution of the KDEL receptor to the Golgi, indicating that Golgi-to-ER transport was perturbed. Electron microscopy revealed an accumulation of COPI-coated membrane profiles close to the Golgi cisternae. Electron tomography showed that the majority of these membrane profiles originate from coated buds, indicating a block in membrane fission. Under these conditions the Golgi-associated pool of ARFGAP1 was reduced, but there was no effect on the binding of coatomer or the membrane fission protein CtBP3/BARS to the Golgi. The addition of 1,2-dioctanoyl-sn-glycerol or the diacylglycerol analogue phorbol 12,13-dibutyrate reversed the effects of endogenous diacylglycerol depletion. Our findings implicate diacylglycerol in the retrograde transport of proteins from Golgi to the ER and suggest that it plays a critical role at a late stage of COPI vesicle formation.

Original languageEnglish (US)
Pages (from-to)3250-3263
Number of pages14
JournalMolecular Biology of the Cell
Volume18
Issue number9
DOIs
StatePublished - Sep 2007
Externally publishedYes

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Coat Protein Complex I
Diglycerides
Membranes
Electron Microscope Tomography
Phorbol 12,13-Dibutyrate
trans-Golgi Network
Secretory Pathway
Electron Microscopy
Carrier Proteins
Membrane Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Fernández-Ulibarri, I., Vilella, M., Lazaro-Dieguez, F., Sarri, E., Martínez, S. E., Jiménez, N., ... Egea, G. (2007). Diacylglycerol is required for the formation of COPI vesicles in the Golgi-to-ER transport pathway. Molecular Biology of the Cell, 18(9), 3250-3263. https://doi.org/10.1091/mbc.E07-04-0334

Diacylglycerol is required for the formation of COPI vesicles in the Golgi-to-ER transport pathway. / Fernández-Ulibarri, Inés; Vilella, Montserrat; Lazaro-Dieguez, Francisco; Sarri, Elisabet; Martínez, Susana E.; Jiménez, Nuria; Claro, Enrique; Mérida, Isabel; Burger, Koert N J; Egea, Gustavo.

In: Molecular Biology of the Cell, Vol. 18, No. 9, 09.2007, p. 3250-3263.

Research output: Contribution to journalArticle

Fernández-Ulibarri, I, Vilella, M, Lazaro-Dieguez, F, Sarri, E, Martínez, SE, Jiménez, N, Claro, E, Mérida, I, Burger, KNJ & Egea, G 2007, 'Diacylglycerol is required for the formation of COPI vesicles in the Golgi-to-ER transport pathway', Molecular Biology of the Cell, vol. 18, no. 9, pp. 3250-3263. https://doi.org/10.1091/mbc.E07-04-0334
Fernández-Ulibarri, Inés ; Vilella, Montserrat ; Lazaro-Dieguez, Francisco ; Sarri, Elisabet ; Martínez, Susana E. ; Jiménez, Nuria ; Claro, Enrique ; Mérida, Isabel ; Burger, Koert N J ; Egea, Gustavo. / Diacylglycerol is required for the formation of COPI vesicles in the Golgi-to-ER transport pathway. In: Molecular Biology of the Cell. 2007 ; Vol. 18, No. 9. pp. 3250-3263.
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