Diacylglycerol is required for the formation of COPI vesicles in the Golgi-to-ER transport pathway

Inés Fernández-Ulibarri, Montserrat Vilella, Francisco Lázaro-Diéguez, Elisabet Sarri, Susana E. Martínez, Nuria Jiménez, Enrique Claro, Isabel Mérida, Koert N.J. Burger, Gustavo Egea

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71 Scopus citations

Abstract

Diacylglycerol is necessary for trans-Golgi network (TGN) to cell surface transport, but its functional relevance in the early secretory pathway is unclear. Although depletion of diacylglycerol did not affect ER-to-Golgi transport, it led to a redistribution of the KDEL receptor to the Golgi, indicating that Golgi-to-ER transport was perturbed. Electron microscopy revealed an accumulation of COPI-coated membrane profiles close to the Golgi cisternae. Electron tomography showed that the majority of these membrane profiles originate from coated buds, indicating a block in membrane fission. Under these conditions the Golgi-associated pool of ARFGAP1 was reduced, but there was no effect on the binding of coatomer or the membrane fission protein CtBP3/BARS to the Golgi. The addition of 1,2-dioctanoyl-sn-glycerol or the diacylglycerol analogue phorbol 12,13-dibutyrate reversed the effects of endogenous diacylglycerol depletion. Our findings implicate diacylglycerol in the retrograde transport of proteins from Golgi to the ER and suggest that it plays a critical role at a late stage of COPI vesicle formation.

Original languageEnglish (US)
Pages (from-to)3250-3263
Number of pages14
JournalMolecular biology of the cell
Volume18
Issue number9
DOIs
StatePublished - Sep 2007

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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    Fernández-Ulibarri, I., Vilella, M., Lázaro-Diéguez, F., Sarri, E., Martínez, S. E., Jiménez, N., Claro, E., Mérida, I., Burger, K. N. J., & Egea, G. (2007). Diacylglycerol is required for the formation of COPI vesicles in the Golgi-to-ER transport pathway. Molecular biology of the cell, 18(9), 3250-3263. https://doi.org/10.1091/mbc.E07-04-0334