Diabetes induced in male transgenic mice by expression of human H-ras oncoprotein in pancreatic β cells

Shimon Efrat, Norman Fleischer, Douglas Hanahan

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Abstract

Transgenic mice expressing an insulin-promoted H-ras hybrid gene in pancreatic β cells developed β-cell degeneration and diabetes. The disease was manifested in male mice by hyperglycemia, glycosuria, and reduced plasma insulin levels, which appeared around 5 months of age and led to premature death. Histological analyses revealed large holes within the islets of Langerhans and a reduced number of β cells. The destruction of the islets was not associated with an obvious inflammatory activity. Ultrastructural analysis showed extensive engorgement in the endoplasmic reticulum of the residual β cells from diabetic males. The females carrying the insulin-promoted ras gene did not manifest any of the physiological abnormalities observed in males and showed only minor histological and ultrastructural changes, even at much greater ages.

Original languageEnglish (US)
Pages (from-to)1779-1783
Number of pages5
JournalMolecular and Cellular Biology
Volume10
Issue number4
StatePublished - 1990

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Oncogene Proteins
Transgenic Mice
ras Genes
Insulin
Glycosuria
Premature Mortality
Islets of Langerhans
Hyperglycemia
Endoplasmic Reticulum
Cell Count

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Diabetes induced in male transgenic mice by expression of human H-ras oncoprotein in pancreatic β cells. / Efrat, Shimon; Fleischer, Norman; Hanahan, Douglas.

In: Molecular and Cellular Biology, Vol. 10, No. 4, 1990, p. 1779-1783.

Research output: Contribution to journalArticle

Efrat, Shimon ; Fleischer, Norman ; Hanahan, Douglas. / Diabetes induced in male transgenic mice by expression of human H-ras oncoprotein in pancreatic β cells. In: Molecular and Cellular Biology. 1990 ; Vol. 10, No. 4. pp. 1779-1783.
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