TY - JOUR
T1 - Dexamethasone downregulates vitamin d receptors in rat kidney, unmasking a high affinity binding site
AU - Gensure, Robert C.
AU - Fish, Jason M.
AU - Walters, Marian R.
PY - 1993/9/15
Y1 - 1993/9/15
N2 - Dexamethasone exerted tissue-specific effects on rat vitamin D receptor levels: upregulation of receptors in intestine, downregulation in kidney, and no effect on receptors in testis, heart, and lung. Scatchard analysis showed selective downregulation of the low affinity receptor site (Kd=0.9 nM) in kidney, thus unmasking a high affinity binding site (Kd=0.05 nM). The single low affinity site in intestine (Kd=0.3-0.7 nM) was upregulated, while the single high affinity site in testis (Kd = 0.07-0.09 nM) was not changed. These results demonstrate the existence of two types of nuclear binding sites in rat kidney and establish that there are similarities between the high affinity binding site in the rat kidney and the single high affinity receptor site in the testis.
AB - Dexamethasone exerted tissue-specific effects on rat vitamin D receptor levels: upregulation of receptors in intestine, downregulation in kidney, and no effect on receptors in testis, heart, and lung. Scatchard analysis showed selective downregulation of the low affinity receptor site (Kd=0.9 nM) in kidney, thus unmasking a high affinity binding site (Kd=0.05 nM). The single low affinity site in intestine (Kd=0.3-0.7 nM) was upregulated, while the single high affinity site in testis (Kd = 0.07-0.09 nM) was not changed. These results demonstrate the existence of two types of nuclear binding sites in rat kidney and establish that there are similarities between the high affinity binding site in the rat kidney and the single high affinity receptor site in the testis.
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U2 - 10.1006/bbrc.1993.2163
DO - 10.1006/bbrc.1993.2163
M3 - Article
C2 - 8396919
AN - SCOPUS:0027304397
SN - 0006-291X
VL - 195
SP - 1139
EP - 1144
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -