Device for in-vivo study of the tumor microenvironment

Waseem Khan Raja, Bojana Gligorijevic, Michael R. Padgen, Douglas Eggers, John Condeelis, James Castracane

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

In-vivo cancer cells create a unique microenvironment which enables their spread to other organs. To understand the tumor microenvironment, special tools and devices are required to monitor the interaction among different cell types as well as the effects of particular chemical gradients. We are reporting on the status of a new device (the NANIVID: NANoIntraVItal Device) that will collect chemotactic cells from the tumor environment. Due to the transparency of this implantable device, direct in-vivo cell imaging both inside and outside the device is possible. The cell collection chamber of the device consists of a micro-electrode system based on patterning of transparent, conducting films that deliver real time data including cell density and dynamics. The current development and testing status of the device will be presented. This will include the modeling of ligand gradient profile results produced from the device and the cell migration in the EGF (epidermal growth factor) gradient created by the device. Further, prototype electrode arrays were designed, fabricated and cells were cultured on the arrays at selected degrees of confluence to measure the device sensitivity. The development path of the NANIVID will be integrated with an existing animal model protocol for in-vivo testing. This will result in a clearer understanding of the dynamics of a tumor's metastatic progression.

Original languageEnglish (US)
Title of host publicationMicrofluidics, BioMEMS, and Medical Microsystems VIII
DOIs
Publication statusPublished - Dec 1 2010
EventMicrofluidics, BioMEMS, and Medical Microsystems VIII - San Francisco, CA, United States
Duration: Jan 25 2010Jan 27 2010

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7593
ISSN (Print)1605-7422

Other

OtherMicrofluidics, BioMEMS, and Medical Microsystems VIII
CountryUnited States
CitySan Francisco, CA
Period1/25/101/27/10

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Keywords

  • Cancer cells migration
  • ITO (indium doped tin oxide)
  • Impedance
  • Microfabrication
  • Numerical modeling

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Raja, W. K., Gligorijevic, B., Padgen, M. R., Eggers, D., Condeelis, J., & Castracane, J. (2010). Device for in-vivo study of the tumor microenvironment. In Microfluidics, BioMEMS, and Medical Microsystems VIII [75930H] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7593). https://doi.org/10.1117/12.841338