Developmental uncoupling between blastoderm and yolk cell in the embryo of the teleost Fundulus

Charles B. Kimmel, David C. Spray, M. V.L. Bennett

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

During cleavage and blastula stages of embryos of the teleost Fundulus heteroclitus all of the cells are both electotonically coupled and dye coupled to one another, as determined by microelectrode impalements and spread of Lucifer Yellow. At about the time that gastrulation begins we observed a specific loss of junctional coupling between the yolk cell and cells of the blastoderm. Passage of Lucifer Yellow between the yolk cell and blastoderm was reduced at stage 12 (late blastula), and not detected at stage 13 and thereafter, although cells of the blastoderm remain dye coupled to one another through gastrula stages. Also, junctional electrical coupling between the yolk cell and blastoderm became substantially reduced at stage 13 and thereafter. The loss of coupling at this specific cell apposition and time and the large size of the yolk cell may prove useful in analyzing the underlying cellular mechanisms.

Original languageEnglish (US)
Pages (from-to)483-487
Number of pages5
JournalDevelopmental Biology
Volume102
Issue number2
DOIs
StatePublished - Apr 1984
Externally publishedYes

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ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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