Developmental Regulation of Nucleolus Size during Drosophila Eye Differentiation

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

When cell cycle withdrawal accompanies terminal differentiation, biosynthesis and cellular growth are likely to change also. In this study, nucleolus size was monitored during cell fate specification in the Drosophila eye imaginal disc using fibrillarin antibody labeling. Nucleolus size is an indicator of ribosome biogenesis and can correlate with cellular growth rate. Nucleolar size was reduced significantly during cell fate specification and differentiation, predominantly as eye disc cells entered a cell cycle arrest that preceded cell fate specification. This reduction in nucleolus size required Dpp and Hh signaling. A transient enlargement of the nucleolus accompanied cell division in the Second Mitotic Wave. Nucleoli continued to diminish in postmitotic cells following fate specification. These results suggest that cellular growth is regulated early in the transition from proliferating progenitor cells to terminal cell fate specification, contemporary with regulation of the cell cycle, and requiring the same extracellular signals.

Original languageEnglish (US)
Article numbere58266
JournalPLoS One
Volume8
Issue number3
DOIs
StatePublished - Mar 5 2013

Fingerprint

cell nucleolus
Drosophila
eyes
Specifications
Cells
cells
cell cycle
Cell Cycle
Growth
Imaginal Discs
Biosynthesis
imaginal discs
Labeling
Cell Cycle Checkpoints
ribosomes
Ribosomes
Cell Division
stem cells
cell division
Stem Cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Developmental Regulation of Nucleolus Size during Drosophila Eye Differentiation. / Baker, Nicholas E.

In: PLoS One, Vol. 8, No. 3, e58266, 05.03.2013.

Research output: Contribution to journalArticle

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