Developmental regulation of hepatitis B surface antigen expression in two lines of hepatitis B virus transgenic mice

J. A. DeLoia, Robert D. Burk, J. D. Gearhart

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Two lines of hepatitis B virus (HBV) transgenic mice, designated G7 and G26, show preferential expression of the 2.1-kilobase hepatitis B surface antigen (HBsAg) RNA transcript in liver and kidney tissues (R.D. Burk, J.A. DeLoia, M.K.ElAwady, and J.D. Gearhart, J. Virol. 62: 649-654, 1988). This transcript was first identified in transgenic mice at gestational day 14 and was detected at similar or increased levels through birth and early development. However, in contrast to 2.1-kilobase HBsAg mRNA levels, HBsAg protein levels in serum decreased shortly after birth. Thereafter, serum HBsAg increased 100-fold to adult levels, with a corresponding 5- to 10-fold increase in HBsAg mRNA levels. In addition, adult males have higher levels of HBsAg in serum than females. HBsAg in serum in males was reduced approximately 50% by surgical castration and was restored to near-normal levels by testosterone supplementation. Since both transgenic lines show similar patterns of gene expression, we suggest that HBsAg gene expression is determined by viral regulatory elements in response to host factors. Whether tissue specificity, developmental regulation, and sexual dimorphism of expression of the exogenous HBV sequences were determined by single or multiple HBV regulatory elements remains to be determined.

Original languageEnglish (US)
Pages (from-to)4069-4073
Number of pages5
JournalJournal of Virology
Volume63
Issue number9
StatePublished - 1989
Externally publishedYes

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Hepatitis B virus
Hepatitis B Surface Antigens
Transgenic Mice
genetically modified organisms
mice
Serum
Parturition
Gene Expression
gene expression
Messenger RNA
Organ Specificity
hepatitis B antigens
response elements
Castration
Response Elements
castration
messenger RNA
Sex Characteristics
sexual dimorphism
testosterone

ASJC Scopus subject areas

  • Immunology

Cite this

Developmental regulation of hepatitis B surface antigen expression in two lines of hepatitis B virus transgenic mice. / DeLoia, J. A.; Burk, Robert D.; Gearhart, J. D.

In: Journal of Virology, Vol. 63, No. 9, 1989, p. 4069-4073.

Research output: Contribution to journalArticle

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