Developmental regulation of epidermal growth factor receptor kinase in rat intestine

John F. Thompson, Merlijn Van Den Berg, Pieter C F Stokkers

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Background/Aims: Intraluminal epidermal growth factor (EGF) may regulate intestinal growth and function. The ontogeny, localization, and phosphorylation of the EGF receptor in rat small intestine were studied. Methods: EGF-receptor phosphorylation was assayed by antiphosphotyrosine Western blot after EGF administration in vivo and EGF incubation to everted sacs in vitro. EGF-receptor abundance and localization were assayed by Western blot and immunofluorescence using anti-EGF-receptor antibodies. Results: In vivo, orogastric EGF enhanced EGF-receptor phosphorylation in newborn rat jejunum and liver. However, intraluminal EGF had no effect on EGF-receptor phosphorylation in adult intestine or liver. In vitro, mucosal EGF stimulated a fourfold increase in EGF-receptor phosphorylation in suckling jejunum but not in weanling or adult jejunum. In Western blot, EGF-receptor abundance was similar in 22-day-old fetal, 8-day-old suckling, and adult jejunum. In both crypt and villus, EGF-receptor was localized to the basolateral membrane but not to the apical membrane in fetal, suckling, and adult jejunum. Conclusions: Mucosal EGF stimulates EGF-receptor phosphorylation in immature but not in mature intestine. These differences are not explained by EGF-receptor abundance or localization and suggest that the greater mucosal permeability of the immature gut may allow EGF to bind to the EGF-receptor in the basolateral membrane to mediate its effects in suckling rat intestine.

Original languageEnglish (US)
Pages (from-to)1278-1287
Number of pages10
JournalGastroenterology
Volume107
Issue number5
DOIs
StatePublished - 1994
Externally publishedYes

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Epidermal Growth Factor Receptor
Intestines
Epidermal Growth Factor
Jejunum
Phosphorylation
Western Blotting
Extraembryonic Membranes
Membranes
Liver
Small Intestine
Fluorescent Antibody Technique
Permeability
Antibodies

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Developmental regulation of epidermal growth factor receptor kinase in rat intestine. / Thompson, John F.; Van Den Berg, Merlijn; Stokkers, Pieter C F.

In: Gastroenterology, Vol. 107, No. 5, 1994, p. 1278-1287.

Research output: Contribution to journalArticle

Thompson, John F. ; Van Den Berg, Merlijn ; Stokkers, Pieter C F. / Developmental regulation of epidermal growth factor receptor kinase in rat intestine. In: Gastroenterology. 1994 ; Vol. 107, No. 5. pp. 1278-1287.
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