Developmental niches for embryonic erythroid cells

Joan Isern, Stuart T. Fraser, Zhiyong He, Margaret H. Baron

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Primitive erythroid cells (EryP) are the first differentiated cell type to be specified during mammalian embryogenesis. EryP arise from a pool of lineage-restricted progenitors in the yolk sac (YS) and then enter the newly formed embryonic circulation to mature in a stepwise, synchronous fashion. Numbering in the millions in the mid-gestation mouse embryo, EryP are the dominant circulating blood cell prior to the rapid generation of adult-type definitive erythroid (EryD) cells in the fetal liver. The identification of maturational events in this lineage presented a significant challenge, as EryD begin to outnumber EryP in the bloodstream from?. E14.5 onwards. We used human epsilon-globin gene regulatory elements to drive lineage-specific expression of a histone-H2B::EGFP fusion protein, allowing us to label the chromatin of EryP during their development and to track and quantify EryP nuclei following their expulsion from the cell. Using this transgenic fluorescent reporter mouse line, we have monitored primitive erythropoiesis in three distinct niches: the YS, where EryP progenitors arise; the circulation, where EryP continue to divide and mature; and the fetal liver, where EryP complete the terminal stages of their differentiation.

Original languageEnglish (US)
Pages (from-to)207-208
Number of pages2
JournalBlood Cells, Molecules, and Diseases
Volume44
Issue number4
DOIs
StatePublished - Apr 2010
Externally publishedYes

Fingerprint

Yolk Sac
Erythroid Cells
epsilon-Globins
Erythropoiesis
Liver
Regulator Genes
Histones
Chromatin
Embryonic Development
Blood Cells
Embryonic Structures
Pregnancy
enhanced green fluorescent protein

Keywords

  • Fetal liver
  • Mouse embryo
  • Primitive erythropoiesis
  • Transgenic mice
  • Yolk sac

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine
  • Hematology
  • Cell Biology

Cite this

Developmental niches for embryonic erythroid cells. / Isern, Joan; Fraser, Stuart T.; He, Zhiyong; Baron, Margaret H.

In: Blood Cells, Molecules, and Diseases, Vol. 44, No. 4, 04.2010, p. 207-208.

Research output: Contribution to journalArticle

Isern, Joan ; Fraser, Stuart T. ; He, Zhiyong ; Baron, Margaret H. / Developmental niches for embryonic erythroid cells. In: Blood Cells, Molecules, and Diseases. 2010 ; Vol. 44, No. 4. pp. 207-208.
@article{6aabc26bb1294162a4f3a5b2c023dbdb,
title = "Developmental niches for embryonic erythroid cells",
abstract = "Primitive erythroid cells (EryP) are the first differentiated cell type to be specified during mammalian embryogenesis. EryP arise from a pool of lineage-restricted progenitors in the yolk sac (YS) and then enter the newly formed embryonic circulation to mature in a stepwise, synchronous fashion. Numbering in the millions in the mid-gestation mouse embryo, EryP are the dominant circulating blood cell prior to the rapid generation of adult-type definitive erythroid (EryD) cells in the fetal liver. The identification of maturational events in this lineage presented a significant challenge, as EryD begin to outnumber EryP in the bloodstream from?. E14.5 onwards. We used human epsilon-globin gene regulatory elements to drive lineage-specific expression of a histone-H2B::EGFP fusion protein, allowing us to label the chromatin of EryP during their development and to track and quantify EryP nuclei following their expulsion from the cell. Using this transgenic fluorescent reporter mouse line, we have monitored primitive erythropoiesis in three distinct niches: the YS, where EryP progenitors arise; the circulation, where EryP continue to divide and mature; and the fetal liver, where EryP complete the terminal stages of their differentiation.",
keywords = "Fetal liver, Mouse embryo, Primitive erythropoiesis, Transgenic mice, Yolk sac",
author = "Joan Isern and Fraser, {Stuart T.} and Zhiyong He and Baron, {Margaret H.}",
year = "2010",
month = "4",
doi = "10.1016/j.bcmd.2010.02.008",
language = "English (US)",
volume = "44",
pages = "207--208",
journal = "Blood Cells, Molecules, and Diseases",
issn = "1079-9796",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - Developmental niches for embryonic erythroid cells

AU - Isern, Joan

AU - Fraser, Stuart T.

AU - He, Zhiyong

AU - Baron, Margaret H.

PY - 2010/4

Y1 - 2010/4

N2 - Primitive erythroid cells (EryP) are the first differentiated cell type to be specified during mammalian embryogenesis. EryP arise from a pool of lineage-restricted progenitors in the yolk sac (YS) and then enter the newly formed embryonic circulation to mature in a stepwise, synchronous fashion. Numbering in the millions in the mid-gestation mouse embryo, EryP are the dominant circulating blood cell prior to the rapid generation of adult-type definitive erythroid (EryD) cells in the fetal liver. The identification of maturational events in this lineage presented a significant challenge, as EryD begin to outnumber EryP in the bloodstream from?. E14.5 onwards. We used human epsilon-globin gene regulatory elements to drive lineage-specific expression of a histone-H2B::EGFP fusion protein, allowing us to label the chromatin of EryP during their development and to track and quantify EryP nuclei following their expulsion from the cell. Using this transgenic fluorescent reporter mouse line, we have monitored primitive erythropoiesis in three distinct niches: the YS, where EryP progenitors arise; the circulation, where EryP continue to divide and mature; and the fetal liver, where EryP complete the terminal stages of their differentiation.

AB - Primitive erythroid cells (EryP) are the first differentiated cell type to be specified during mammalian embryogenesis. EryP arise from a pool of lineage-restricted progenitors in the yolk sac (YS) and then enter the newly formed embryonic circulation to mature in a stepwise, synchronous fashion. Numbering in the millions in the mid-gestation mouse embryo, EryP are the dominant circulating blood cell prior to the rapid generation of adult-type definitive erythroid (EryD) cells in the fetal liver. The identification of maturational events in this lineage presented a significant challenge, as EryD begin to outnumber EryP in the bloodstream from?. E14.5 onwards. We used human epsilon-globin gene regulatory elements to drive lineage-specific expression of a histone-H2B::EGFP fusion protein, allowing us to label the chromatin of EryP during their development and to track and quantify EryP nuclei following their expulsion from the cell. Using this transgenic fluorescent reporter mouse line, we have monitored primitive erythropoiesis in three distinct niches: the YS, where EryP progenitors arise; the circulation, where EryP continue to divide and mature; and the fetal liver, where EryP complete the terminal stages of their differentiation.

KW - Fetal liver

KW - Mouse embryo

KW - Primitive erythropoiesis

KW - Transgenic mice

KW - Yolk sac

UR - http://www.scopus.com/inward/record.url?scp=77950296180&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77950296180&partnerID=8YFLogxK

U2 - 10.1016/j.bcmd.2010.02.008

DO - 10.1016/j.bcmd.2010.02.008

M3 - Article

C2 - 20181503

AN - SCOPUS:77950296180

VL - 44

SP - 207

EP - 208

JO - Blood Cells, Molecules, and Diseases

JF - Blood Cells, Molecules, and Diseases

SN - 1079-9796

IS - 4

ER -