Abstract
Murine light chain 3 (LC3) exists as two isoforms, LC3α and β: LC3β is an RNA-binding protein that enhances fibronectin (FN) mRNA translation, and is also a marker of autophagy. We report embryonic expression patterns for LC3α and LC3β, with some overlap but notable differences in the brain, and in tissues of non-neuronal origin. LC3β knockout (-/-) mice develop normally without a compensatory increase in LC3α. LC3β-/- embryonic fibroblasts (MEFs) exhibit reduced FN synthesis but maintain wild type (WT) levels of FN protein. No significant changes in proteins associated with FN turnover, i.e., caveolin-1, LRP-1, or matrix metalloproteinases were identified. Autophagosomes form in amino acid-starved LC3β-/-MEFs, and Caesarean-delivered pups survive as long as WT pups without an increase in LC3-related proteins linked to autophagy. These results suggest novel compensatory mechanisms for loss of LC3β, ensuring proper FN accumulation and autophagy during fetal and neonatal life.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 187-195 |
| Number of pages | 9 |
| Journal | Developmental Dynamics |
| Volume | 237 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2008 |
| Externally published | Yes |
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Keywords
- Autophagy
- Fibronectin
- LC3
- RNA-binding protein
- Transgenic mouse
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology
Cite this
Developmental expression of LC3α and β : Absence of fibronectin or autophagy phenotype in LC3β knockout mice. / Cann, Gordon M.; Guignabert, Christophe; Ying, Lihua; Deshpande, Niru; Bekker, Janine M.; Wang, Lingli; Zhou, Bin; Rabinovitch, Marlene.
In: Developmental Dynamics, Vol. 237, No. 1, 01.2008, p. 187-195.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Developmental expression of LC3α and β
T2 - Absence of fibronectin or autophagy phenotype in LC3β knockout mice
AU - Cann, Gordon M.
AU - Guignabert, Christophe
AU - Ying, Lihua
AU - Deshpande, Niru
AU - Bekker, Janine M.
AU - Wang, Lingli
AU - Zhou, Bin
AU - Rabinovitch, Marlene
PY - 2008/1
Y1 - 2008/1
N2 - Murine light chain 3 (LC3) exists as two isoforms, LC3α and β: LC3β is an RNA-binding protein that enhances fibronectin (FN) mRNA translation, and is also a marker of autophagy. We report embryonic expression patterns for LC3α and LC3β, with some overlap but notable differences in the brain, and in tissues of non-neuronal origin. LC3β knockout (-/-) mice develop normally without a compensatory increase in LC3α. LC3β-/- embryonic fibroblasts (MEFs) exhibit reduced FN synthesis but maintain wild type (WT) levels of FN protein. No significant changes in proteins associated with FN turnover, i.e., caveolin-1, LRP-1, or matrix metalloproteinases were identified. Autophagosomes form in amino acid-starved LC3β-/-MEFs, and Caesarean-delivered pups survive as long as WT pups without an increase in LC3-related proteins linked to autophagy. These results suggest novel compensatory mechanisms for loss of LC3β, ensuring proper FN accumulation and autophagy during fetal and neonatal life.
AB - Murine light chain 3 (LC3) exists as two isoforms, LC3α and β: LC3β is an RNA-binding protein that enhances fibronectin (FN) mRNA translation, and is also a marker of autophagy. We report embryonic expression patterns for LC3α and LC3β, with some overlap but notable differences in the brain, and in tissues of non-neuronal origin. LC3β knockout (-/-) mice develop normally without a compensatory increase in LC3α. LC3β-/- embryonic fibroblasts (MEFs) exhibit reduced FN synthesis but maintain wild type (WT) levels of FN protein. No significant changes in proteins associated with FN turnover, i.e., caveolin-1, LRP-1, or matrix metalloproteinases were identified. Autophagosomes form in amino acid-starved LC3β-/-MEFs, and Caesarean-delivered pups survive as long as WT pups without an increase in LC3-related proteins linked to autophagy. These results suggest novel compensatory mechanisms for loss of LC3β, ensuring proper FN accumulation and autophagy during fetal and neonatal life.
KW - Autophagy
KW - Fibronectin
KW - LC3
KW - RNA-binding protein
KW - Transgenic mouse
UR - http://www.scopus.com/inward/record.url?scp=38149098485&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=38149098485&partnerID=8YFLogxK
U2 - 10.1002/dvdy.21392
DO - 10.1002/dvdy.21392
M3 - Article
C2 - 18069693
AN - SCOPUS:38149098485
VL - 237
SP - 187
EP - 195
JO - Developmental Dynamics
JF - Developmental Dynamics
SN - 1058-8388
IS - 1
ER -