Development of recombinant scFv-antibodies against diphtheria toxin using phage display system

E. S. Oleǐnik, A. A. Kaberniuk, D. A. Burkaleva, S. I. Romaniuk, D. V. Kolibo, A. O. Shepeliakovskaia, A. G. Laman, S. V. Komisarenko

Research output: Contribution to journalArticle

Abstract

Phage display technology is an effective approach to the development of the next generation of immunodiagnostic reagents. Naive murine phage display a library of single-chain variable antibodies (scFv) was used to isolate scFv recognizing the diphtheria toxin, an important diagnostic antigen of diphtheria. The diphtheria toxin B subunit-binding clone with affinity constant of 1.13 x 10(7) M(-1) was selected. scFv preserved activity on storage in the course of 8 months.

Original languageEnglish (US)
Pages (from-to)91-97
Number of pages7
JournalUkrainskiǐ biokhimicheskiǐ zhurnal
Volume79
Issue number5
StatePublished - Sep 2007
Externally publishedYes

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Diphtheria Toxin
Single-Chain Antibodies
Bacteriophages
Display devices
Diphtheria
Clone Cells
Technology
Antigens

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Oleǐnik, E. S., Kaberniuk, A. A., Burkaleva, D. A., Romaniuk, S. I., Kolibo, D. V., Shepeliakovskaia, A. O., ... Komisarenko, S. V. (2007). Development of recombinant scFv-antibodies against diphtheria toxin using phage display system. Ukrainskiǐ biokhimicheskiǐ zhurnal, 79(5), 91-97.

Development of recombinant scFv-antibodies against diphtheria toxin using phage display system. / Oleǐnik, E. S.; Kaberniuk, A. A.; Burkaleva, D. A.; Romaniuk, S. I.; Kolibo, D. V.; Shepeliakovskaia, A. O.; Laman, A. G.; Komisarenko, S. V.

In: Ukrainskiǐ biokhimicheskiǐ zhurnal, Vol. 79, No. 5, 09.2007, p. 91-97.

Research output: Contribution to journalArticle

Oleǐnik, ES, Kaberniuk, AA, Burkaleva, DA, Romaniuk, SI, Kolibo, DV, Shepeliakovskaia, AO, Laman, AG & Komisarenko, SV 2007, 'Development of recombinant scFv-antibodies against diphtheria toxin using phage display system', Ukrainskiǐ biokhimicheskiǐ zhurnal, vol. 79, no. 5, pp. 91-97.
Oleǐnik ES, Kaberniuk AA, Burkaleva DA, Romaniuk SI, Kolibo DV, Shepeliakovskaia AO et al. Development of recombinant scFv-antibodies against diphtheria toxin using phage display system. Ukrainskiǐ biokhimicheskiǐ zhurnal. 2007 Sep;79(5):91-97.
Oleǐnik, E. S. ; Kaberniuk, A. A. ; Burkaleva, D. A. ; Romaniuk, S. I. ; Kolibo, D. V. ; Shepeliakovskaia, A. O. ; Laman, A. G. ; Komisarenko, S. V. / Development of recombinant scFv-antibodies against diphtheria toxin using phage display system. In: Ukrainskiǐ biokhimicheskiǐ zhurnal. 2007 ; Vol. 79, No. 5. pp. 91-97.
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