Development of modified siRNA molecules incorporating 5-fluoro-2′- deoxyuridine residues to enhance cytotoxicity

Shao Yu Wu, Tian Min Chen, William H. Gmeiner, Edward Chu, John C. Schmitz

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Therapeutic small interfering RNAs (siRNAs) are composed of chemically modified nucleotides, which enhance RNA stability and increase affinity in Watson-Crick base pairing. However, the precise fate of such modified nucleotides once the siRNA is degraded within the cell is unknown. Previously, we demonstrated that deoxythymidine release from degraded siRNAs reversed the cytotoxicity of thymidylate synthase (TS)-targeted siRNAs and other TS inhibitor compounds. We hypothesized that siRNAs could be designed with specific nucleoside analogues that, once released, would enhance siRNA cytotoxicity. TS-targeted siRNAs were designed that contained 5-fluoro-2′-deoxyuridine (FdU) moieties at various locations within the siRNA. After transfection, these siRNAs suppressed TS protein and messenger RNA expression with different efficiencies depending on the location of the FdU modification. FdU was rapidly released from the siRNA as evidenced by formation of the covalent inhibitory ternary complex formed between TS protein and the FdU metabolite, FdUMP. These modified siRNAs exhibited 10-100-fold greater cytotoxicity and induced multiple DNA damage repair and apoptotic pathways when compared with control siRNAs. The strategy of designing siRNA molecules that incorporate cytotoxic nucleosides represents a potentially novel drug development approach for the treatment of cancer and other human diseases.

Original languageEnglish (US)
Pages (from-to)4650-4659
Number of pages10
JournalNucleic acids research
Volume41
Issue number8
DOIs
StatePublished - Apr 2013
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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