Development of a rapid serological assay for the diagnosis of strongyloidiasis using a novel diffraction-based biosensor technology

Brian J. Pak, Fabio Vasquez-Camargo, Evgeniya Kalinichenko, Peter L. Chiodini, Thomas B. Nutman, Herbert B. Tanowitz, Isabel McAuliffe, Patricia Wilkins, Paul T. Smith, Brian J. Ward, Michael D. Libman, Momar Ndao

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

BACKGROUND: Strongyloidiasis is a persistent human parasitic infection caused by the intestinal nematode, Strongyloides stercoralis. The parasite has a world-wide distribution, particularly in tropical and subtropical regions with poor sanitary conditions. Since individuals with strongyloidiasis are typically asymptomatic, the infection can persist for decades without detection. Problems arise when individuals with unrecognized S. stercoralis infection are immunosuppressed, which can lead to hyper-infection syndrome and disseminated disease with an associated high mortality if untreated. Therefore a rapid, sensitive and easy to use method of diagnosing Strongyloides infection may improve the clinical management of this disease.

METHODOLOGY/PRINCIPAL FINDINGS: An immunological assay for diagnosing strongyloidiasis was developed on a novel diffraction-based optical bionsensor technology. The test employs a 31-kDa recombinant antigen called NIE derived from Strongyloides stercoralis L3-stage larvae. Assay performance was tested using retrospectively collected sera from patients with parasitologically confirmed strongyloidiasis and control sera from healthy individuals or those with other parasitoses including schistosomiasis, trichinosis, echinococcosis or amebiasis who were seronegative using the NIE ELISA assay. If we consider the control group as the true negative group, the assay readily differentiated S. stercoralis-infected patients from controls detecting 96.3% of the positive cases, and with no cross reactivity observed in the control group These results were in excellent agreement (κ = 0.98) with results obtained by an NIE-based enzyme-linked immunosorbent assay (ELISA). A further 44 sera from patients with suspected S. stercoralis infection were analyzed and showed 91% agreement with the NIE ELISA.

CONCLUSIONS/SIGNIFICANCE: In summary, this test provides high sensitivity detection of serum IgG against the NIE Strongyloides antigen. The assay is easy to perform and provides results in less than 30 minutes, making this platform amenable to rapid near-patient screening with minimal technical expertise.

Original languageEnglish (US)
Pages (from-to)e3002
JournalPLoS Neglected Tropical Diseases
Volume8
Issue number8
DOIs
StatePublished - Aug 1 2014

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Strongyloidiasis
Strongyloides stercoralis
Biosensing Techniques
Technology
Strongyloides
Enzyme-Linked Immunosorbent Assay
Infection
Serum
Professional Competence
Trichinellosis
Antigens
Amebiasis
Control Groups
Parasitic Diseases
Asymptomatic Infections
Schistosomiasis
Echinococcosis
Disease Management
Larva
Parasites

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Pak, B. J., Vasquez-Camargo, F., Kalinichenko, E., Chiodini, P. L., Nutman, T. B., Tanowitz, H. B., ... Ndao, M. (2014). Development of a rapid serological assay for the diagnosis of strongyloidiasis using a novel diffraction-based biosensor technology. PLoS Neglected Tropical Diseases, 8(8), e3002. https://doi.org/10.1371/journal.pntd.0003002

Development of a rapid serological assay for the diagnosis of strongyloidiasis using a novel diffraction-based biosensor technology. / Pak, Brian J.; Vasquez-Camargo, Fabio; Kalinichenko, Evgeniya; Chiodini, Peter L.; Nutman, Thomas B.; Tanowitz, Herbert B.; McAuliffe, Isabel; Wilkins, Patricia; Smith, Paul T.; Ward, Brian J.; Libman, Michael D.; Ndao, Momar.

In: PLoS Neglected Tropical Diseases, Vol. 8, No. 8, 01.08.2014, p. e3002.

Research output: Contribution to journalArticle

Pak, BJ, Vasquez-Camargo, F, Kalinichenko, E, Chiodini, PL, Nutman, TB, Tanowitz, HB, McAuliffe, I, Wilkins, P, Smith, PT, Ward, BJ, Libman, MD & Ndao, M 2014, 'Development of a rapid serological assay for the diagnosis of strongyloidiasis using a novel diffraction-based biosensor technology', PLoS Neglected Tropical Diseases, vol. 8, no. 8, pp. e3002. https://doi.org/10.1371/journal.pntd.0003002
Pak, Brian J. ; Vasquez-Camargo, Fabio ; Kalinichenko, Evgeniya ; Chiodini, Peter L. ; Nutman, Thomas B. ; Tanowitz, Herbert B. ; McAuliffe, Isabel ; Wilkins, Patricia ; Smith, Paul T. ; Ward, Brian J. ; Libman, Michael D. ; Ndao, Momar. / Development of a rapid serological assay for the diagnosis of strongyloidiasis using a novel diffraction-based biosensor technology. In: PLoS Neglected Tropical Diseases. 2014 ; Vol. 8, No. 8. pp. e3002.
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abstract = "BACKGROUND: Strongyloidiasis is a persistent human parasitic infection caused by the intestinal nematode, Strongyloides stercoralis. The parasite has a world-wide distribution, particularly in tropical and subtropical regions with poor sanitary conditions. Since individuals with strongyloidiasis are typically asymptomatic, the infection can persist for decades without detection. Problems arise when individuals with unrecognized S. stercoralis infection are immunosuppressed, which can lead to hyper-infection syndrome and disseminated disease with an associated high mortality if untreated. Therefore a rapid, sensitive and easy to use method of diagnosing Strongyloides infection may improve the clinical management of this disease.METHODOLOGY/PRINCIPAL FINDINGS: An immunological assay for diagnosing strongyloidiasis was developed on a novel diffraction-based optical bionsensor technology. The test employs a 31-kDa recombinant antigen called NIE derived from Strongyloides stercoralis L3-stage larvae. Assay performance was tested using retrospectively collected sera from patients with parasitologically confirmed strongyloidiasis and control sera from healthy individuals or those with other parasitoses including schistosomiasis, trichinosis, echinococcosis or amebiasis who were seronegative using the NIE ELISA assay. If we consider the control group as the true negative group, the assay readily differentiated S. stercoralis-infected patients from controls detecting 96.3{\%} of the positive cases, and with no cross reactivity observed in the control group These results were in excellent agreement (κ = 0.98) with results obtained by an NIE-based enzyme-linked immunosorbent assay (ELISA). A further 44 sera from patients with suspected S. stercoralis infection were analyzed and showed 91{\%} agreement with the NIE ELISA.CONCLUSIONS/SIGNIFICANCE: In summary, this test provides high sensitivity detection of serum IgG against the NIE Strongyloides antigen. The assay is easy to perform and provides results in less than 30 minutes, making this platform amenable to rapid near-patient screening with minimal technical expertise.",
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