TY - JOUR
T1 - Development and migration of plasma cells in the mouse lymph node
AU - Fooksman, David R.
AU - Schwickert, Tanja A.
AU - Victora, Gabriel D.
AU - Dustin, Michael L.
AU - Nussenzweig, Michel C.
AU - Skokos, Dimitris
N1 - Funding Information:
We would like to thank E. Meffre for Blimp-1-YFP mice, T. Starr for making ICAM-1 reagents, and the EM facility at Rockefeller University for help preparing and imaging cells. This work was supported by National Institutes of Health grants R01AI072529 (M.C.N. and M.L.D.), R56AI44931 (M.L.D.), and CA009161-34 (D.R.F.) and a Schering fellowship (T.S.).
PY - 2010/7
Y1 - 2010/7
N2 - In this study, we imaged the differentiation and migratory behavior of nascent plasma cells (PCs) in mouse lymph nodes by intravital microscopy. Pre-PCs exhibited a unique migration pattern characterized by long, linear paths that were randomly oriented. Although chemotaxis via Gαi coupled-receptors has been implicated in PC migration, treatment with Pertussis toxin (Ptx), which ablates these signals, did not prevent movement of pre-PCs while it arrested other lymphocytes. In vitro, pre-PCs displayed processive amoeboid locomotion on surfaces coated with integrin ligand, whereas fully differentiated PCs moved slowly or were arrested. Both PC arrest and differentiation occurred in the medullary cords. Ptx treatment before PC differentiation blocked their accumulation in the medullary cords but pre-PCs still differentiated in other lymph node regions. Taken together, we suggest pre-PCs undergo a persistent random walk to find the medullary cords, where localized chemokines help retain these cells until they undergo differentiation and arrest in situ.
AB - In this study, we imaged the differentiation and migratory behavior of nascent plasma cells (PCs) in mouse lymph nodes by intravital microscopy. Pre-PCs exhibited a unique migration pattern characterized by long, linear paths that were randomly oriented. Although chemotaxis via Gαi coupled-receptors has been implicated in PC migration, treatment with Pertussis toxin (Ptx), which ablates these signals, did not prevent movement of pre-PCs while it arrested other lymphocytes. In vitro, pre-PCs displayed processive amoeboid locomotion on surfaces coated with integrin ligand, whereas fully differentiated PCs moved slowly or were arrested. Both PC arrest and differentiation occurred in the medullary cords. Ptx treatment before PC differentiation blocked their accumulation in the medullary cords but pre-PCs still differentiated in other lymph node regions. Taken together, we suggest pre-PCs undergo a persistent random walk to find the medullary cords, where localized chemokines help retain these cells until they undergo differentiation and arrest in situ.
KW - Cellimmuno
KW - Molimmuno
UR - http://www.scopus.com/inward/record.url?scp=77954895311&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77954895311&partnerID=8YFLogxK
U2 - 10.1016/j.immuni.2010.06.015
DO - 10.1016/j.immuni.2010.06.015
M3 - Article
C2 - 20619695
AN - SCOPUS:77954895311
SN - 1074-7613
VL - 33
SP - 118
EP - 127
JO - Immunity
JF - Immunity
IS - 1
ER -
