Development and characterization of a variant GC cell line with L-triiodothyronine-independent growth and growth hormone production

Y. Halperin, L. E. Shapiro, Martin I. Surks

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Abstract

To facilitate studies of cell growth regulation by T3, we developed a variant GC cell line (V-GC) characterized by normal growth in T3-depleted (-T3) medium. The doubling time (dt) of V-GC cells was 28.8 h (-T3) and 28.0 h (+0.2 nM T3), respectively, whereas the dt of the parent GC cells, 24.0 h (+0.2 nM T3), increased to more than 100 h (-T3). The dt of V-GC cell was unaffected even by maximal T3 (5 nM). Cell protein (micrograms) per μg DNA increased in GC cells in a T3 concentration-dependent manner, whereas V-GC cell protein was unaffected by T3. GH production appeared partially independent of T3 in V-GC cells. GH production (nanograms per 106/h) in V-GC cells maintained for 3 months in -T3 medium was 3.3- to 4.6-fold greater than that in GC cells after 4 days in -T3 medium (P < 0.001). Addition of T3 resulted in similar maximal GH production in both cell lines. The binding capacity and K(a) of nuclear T3 receptors were similar in GC and V-GC cultures, and receptor down-regulation in response to added T3 occurred similarly in both cultures. Lastly, studies employing conditioned medium indicated that T3-independent growth of V-GC cells did not result from production of an autocrine growth factor. Our findings raise the possibility that overexpression of a transacting cell-specific gene-regulating protein that variably affects thyroid hormone-dependent genes may account for the phenotype of the V-GC cultures.

Original languageEnglish (US)
Pages (from-to)778-784
Number of pages7
JournalEndocrinology
Volume125
Issue number2
StatePublished - 1989

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Triiodothyronine
Growth Hormone
Cell Line
Growth
Cell Culture Techniques
Thyroid Hormone Receptors
Proteins
Cytoplasmic and Nuclear Receptors
Conditioned Culture Medium
Thyroid Hormones
Intercellular Signaling Peptides and Proteins
Down-Regulation
Phenotype

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Development and characterization of a variant GC cell line with L-triiodothyronine-independent growth and growth hormone production. / Halperin, Y.; Shapiro, L. E.; Surks, Martin I.

In: Endocrinology, Vol. 125, No. 2, 1989, p. 778-784.

Research output: Contribution to journalArticle

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