Developing a time-resolved flow cytometer for fluorescence lifetime measurements of near-infrared fluorescent proteins

Zhihua Yang, Daria Shcherbakova, Vladislav Verkhusha, Jessica P. Houston

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Excited state fluorescence lifetimes of near-infrared fluorescent proteins iRFP682 and iRFP713 engineered from bacterial phytochromes were measured with a time-resolved flow cytometer. Average lifetimes ranged between 0.5 and 1.0 ns for n=5000 bacterial cells. We anticipate cell sorting for screening cells expressing mutated iRFP variants of high quantum yield.

Original languageEnglish (US)
Title of host publication2016 Conference on Lasers and Electro-Optics, CLEO 2016
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781943580118
StatePublished - Dec 16 2016
Event2016 Conference on Lasers and Electro-Optics, CLEO 2016 - San Jose, United States
Duration: Jun 5 2016Jun 10 2016

Other

Other2016 Conference on Lasers and Electro-Optics, CLEO 2016
CountryUnited States
CitySan Jose
Period6/5/166/10/16

Fingerprint

phytochrome
Fluorescence
Cells
Phytochrome
Infrared radiation
proteins
Proteins
life (durability)
fluorescence
Quantum yield
classifying
Sorting
Excited states
Screening
screening
cells
excitation

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Electrical and Electronic Engineering

Cite this

Yang, Z., Shcherbakova, D., Verkhusha, V., & Houston, J. P. (2016). Developing a time-resolved flow cytometer for fluorescence lifetime measurements of near-infrared fluorescent proteins. In 2016 Conference on Lasers and Electro-Optics, CLEO 2016 [7789260] Institute of Electrical and Electronics Engineers Inc..

Developing a time-resolved flow cytometer for fluorescence lifetime measurements of near-infrared fluorescent proteins. / Yang, Zhihua; Shcherbakova, Daria; Verkhusha, Vladislav; Houston, Jessica P.

2016 Conference on Lasers and Electro-Optics, CLEO 2016. Institute of Electrical and Electronics Engineers Inc., 2016. 7789260.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yang, Z, Shcherbakova, D, Verkhusha, V & Houston, JP 2016, Developing a time-resolved flow cytometer for fluorescence lifetime measurements of near-infrared fluorescent proteins. in 2016 Conference on Lasers and Electro-Optics, CLEO 2016., 7789260, Institute of Electrical and Electronics Engineers Inc., 2016 Conference on Lasers and Electro-Optics, CLEO 2016, San Jose, United States, 6/5/16.
Yang Z, Shcherbakova D, Verkhusha V, Houston JP. Developing a time-resolved flow cytometer for fluorescence lifetime measurements of near-infrared fluorescent proteins. In 2016 Conference on Lasers and Electro-Optics, CLEO 2016. Institute of Electrical and Electronics Engineers Inc. 2016. 7789260
Yang, Zhihua ; Shcherbakova, Daria ; Verkhusha, Vladislav ; Houston, Jessica P. / Developing a time-resolved flow cytometer for fluorescence lifetime measurements of near-infrared fluorescent proteins. 2016 Conference on Lasers and Electro-Optics, CLEO 2016. Institute of Electrical and Electronics Engineers Inc., 2016.
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