Developing a time-resolved flow cytometer for fluorescence lifetime measurements of near-infrared fluorescent proteins

Zhihua Yang, Daria M. Shcherbakova, Vladislav V. Verkhusha, Jessica P. Houston

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Excited state fluorescence lifetimes of near-infrared fluorescent proteins iRFP682 and iRFP713 engineered from bacterial phytochromes were measured with a time-resolved flow cytometer. Average lifetimes ranged between 0.5 and 1.0 ns for n=5000 bacterial cells. We anticipate cell sorting for screening cells expressing mutated iRFP variants of high quantum yield.

Original languageEnglish (US)
Title of host publication2016 Conference on Lasers and Electro-Optics, CLEO 2016
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781943580118
DOIs
StatePublished - Dec 16 2016
Event2016 Conference on Lasers and Electro-Optics, CLEO 2016 - San Jose, United States
Duration: Jun 5 2016Jun 10 2016

Publication series

Name2016 Conference on Lasers and Electro-Optics, CLEO 2016

Other

Other2016 Conference on Lasers and Electro-Optics, CLEO 2016
CountryUnited States
CitySan Jose
Period6/5/166/10/16

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Electrical and Electronic Engineering

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    Yang, Z., Shcherbakova, D. M., Verkhusha, V. V., & Houston, J. P. (2016). Developing a time-resolved flow cytometer for fluorescence lifetime measurements of near-infrared fluorescent proteins. In 2016 Conference on Lasers and Electro-Optics, CLEO 2016 [7789260] (2016 Conference on Lasers and Electro-Optics, CLEO 2016). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1364/cleo_si.2016.sw4g.1