Determination of the structure of the recombinant T = 1 capsid of Sesbania mosaic virus

V. Sangita, S. Parthasarathy, S. Toma, G. L. Lokesh, T. D S Gowri, P. S. Satheshkumar, H. S. Savithri, M. R N Murthy

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The recombinant coat protein (CP) of Sesbania mosaic virus lacking segments of different lengths from the N-terminus expressed in E. coli was shown to self-assemble into a variety of distinct capsids encapsidating 23S rRNA from the host and CP mRNA in vivo. Particles with 60 copies (T = 1) of protein subunits were observed when protein lacking 65 amino acids from the N-terminus was expressed. This recombinant protein possesses the sequence corresponding to the S-domain of the native, T = 3 icosahedral particles but lacks the β-annulus, the βA strand (residues 67-70) and the arginine-rich ARM motif (residues 28-36). Purified T = 1 particles crystallized in the monoclinic snace group P21 with cell parameters of a = 188.4 Å, b = 194.6 Å, c = 272.1 Å and β = 92.6°. The structure of the T = 1 particles was determined by X-ray diffraction at 3.0 Å resolution. As expected, the polypeptide fold of the subunit closely resembles that of the S-domain of the native virus. The recombinant particles bind calcium ions in a manner indistinguishable from that of the native capsids. The structure reveals the major differences in the quaternary organization responsible for the formation of T = 1 against T = 3 particles.

Original languageEnglish (US)
Pages (from-to)1123-1131
Number of pages9
JournalCurrent Science
Volume82
Issue number9
StatePublished - May 10 2002
Externally publishedYes

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Sesbania mosaic virus
Sesbania
Mosaic Viruses
capsid
Capsid
Capsid Proteins
Recombinant Proteins
Protein Subunits
X-Ray Diffraction
Arginine
Ions
Escherichia coli
Viruses
Calcium
coat proteins
Amino Acids
recombinant proteins
Messenger RNA
Peptides
Proteins

ASJC Scopus subject areas

  • General
  • Agricultural and Biological Sciences(all)

Cite this

Sangita, V., Parthasarathy, S., Toma, S., Lokesh, G. L., Gowri, T. D. S., Satheshkumar, P. S., ... Murthy, M. R. N. (2002). Determination of the structure of the recombinant T = 1 capsid of Sesbania mosaic virus. Current Science, 82(9), 1123-1131.

Determination of the structure of the recombinant T = 1 capsid of Sesbania mosaic virus. / Sangita, V.; Parthasarathy, S.; Toma, S.; Lokesh, G. L.; Gowri, T. D S; Satheshkumar, P. S.; Savithri, H. S.; Murthy, M. R N.

In: Current Science, Vol. 82, No. 9, 10.05.2002, p. 1123-1131.

Research output: Contribution to journalArticle

Sangita, V, Parthasarathy, S, Toma, S, Lokesh, GL, Gowri, TDS, Satheshkumar, PS, Savithri, HS & Murthy, MRN 2002, 'Determination of the structure of the recombinant T = 1 capsid of Sesbania mosaic virus', Current Science, vol. 82, no. 9, pp. 1123-1131.
Sangita V, Parthasarathy S, Toma S, Lokesh GL, Gowri TDS, Satheshkumar PS et al. Determination of the structure of the recombinant T = 1 capsid of Sesbania mosaic virus. Current Science. 2002 May 10;82(9):1123-1131.
Sangita, V. ; Parthasarathy, S. ; Toma, S. ; Lokesh, G. L. ; Gowri, T. D S ; Satheshkumar, P. S. ; Savithri, H. S. ; Murthy, M. R N. / Determination of the structure of the recombinant T = 1 capsid of Sesbania mosaic virus. In: Current Science. 2002 ; Vol. 82, No. 9. pp. 1123-1131.
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