Determination of the structural environment of the tyrosyl radical in prostaglandin H2 synthase-1: A high frequency ENDOR/EPR study

John C. Wilson, Gang Wu, Ah Lim Tsai, Gary J. Gerfen

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The catalytically active tyrosyl radical which gives rise to the "wide doublet" (WD1) signal in ovine Prostaglandin H2 Synthase-1 has been studied using high frequency (HF) pulsed ENDOR and EPR. A hydrogen-bonded deuteron was directly detected in HFENDOR (130 GHz) spectra of 1H2O/2H2O-exchanged samples. The HFENDOR spectral simulations required a distribution in hydrogen bond distances to achieve proper fits. This range of distances was consistent with that used to model the distribution in gX values detected in pulsed HFEPR spectra. Possible hydrogen-bonding partners, as well as implications regarding the mechanism of self-inactivation for PGHS, are discussed.

Original languageEnglish (US)
Pages (from-to)1618-1619
Number of pages2
JournalJournal of the American Chemical Society
Volume127
Issue number6
DOIs
StatePublished - Feb 16 2005

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Prostaglandin H2
Electron Spin Resonance Spectroscopy
Prostaglandin-Endoperoxide Synthases
Paramagnetic resonance
Hydrogen
Hydrogen bonds
Deuterium
Hydrogen Bonding
Sheep

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Determination of the structural environment of the tyrosyl radical in prostaglandin H2 synthase-1 : A high frequency ENDOR/EPR study. / Wilson, John C.; Wu, Gang; Tsai, Ah Lim; Gerfen, Gary J.

In: Journal of the American Chemical Society, Vol. 127, No. 6, 16.02.2005, p. 1618-1619.

Research output: Contribution to journalArticle

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