Determination of the leukaemogenicity of a murine retrovirus by sequences within the long terminal repeat

Jack Lenz, Daniel Celander, Robert L. Crowther, Roberto Patarca, Dennis W. Perkins, William A. Haseltine

Research output: Contribution to journalArticle

167 Citations (Scopus)

Abstract

Although the murine retrovirus SL3-3 is highly leukaemo-genic 1,2, in both the structure of its genome and in its properties of replication in tissue culture it closely resembles the non-leukaemogenic retrovirus Akv (refs 3, 4). An.earlier investigation of the properties of recombinant SL3-3-Akv viruses localized the major determinant of leukaemogenicity outside the env gene, in a region of the viral genome that includes the gag gene and the noncoding long terminal repeat (LTR)4. To localize the determinant of SL3-3's leukaemogenicity more precisely we have now construced a recombinant provirus containing the LTR of SL3-3 and the coding region of Akv. The leukaemogenicity of these recombinants demonstrates that the determinant of leukaemogenicity ties within the SL3-3 LTR. Nucleotide sequencing of the LTRs of SL3-3 and Akv shows that they differ by a set of changes in the region thought to contain a transcriptional enhancer element. We suggest that enhancer region sequences are the major determinants of leukaemogenicity in these viruses.

Original languageEnglish (US)
Pages (from-to)467-470
Number of pages4
JournalNature
Volume308
Issue number5958
DOIs
StatePublished - 1984
Externally publishedYes

Fingerprint

Terminal Repeat Sequences
Retroviridae
gag Genes
Viruses
env Genes
Proviruses
Viral Genome
Nucleotides
Genome

ASJC Scopus subject areas

  • General

Cite this

Lenz, J., Celander, D., Crowther, R. L., Patarca, R., Perkins, D. W., & Haseltine, W. A. (1984). Determination of the leukaemogenicity of a murine retrovirus by sequences within the long terminal repeat. Nature, 308(5958), 467-470. https://doi.org/10.1038/308467a0

Determination of the leukaemogenicity of a murine retrovirus by sequences within the long terminal repeat. / Lenz, Jack; Celander, Daniel; Crowther, Robert L.; Patarca, Roberto; Perkins, Dennis W.; Haseltine, William A.

In: Nature, Vol. 308, No. 5958, 1984, p. 467-470.

Research output: Contribution to journalArticle

Lenz, J, Celander, D, Crowther, RL, Patarca, R, Perkins, DW & Haseltine, WA 1984, 'Determination of the leukaemogenicity of a murine retrovirus by sequences within the long terminal repeat', Nature, vol. 308, no. 5958, pp. 467-470. https://doi.org/10.1038/308467a0
Lenz, Jack ; Celander, Daniel ; Crowther, Robert L. ; Patarca, Roberto ; Perkins, Dennis W. ; Haseltine, William A. / Determination of the leukaemogenicity of a murine retrovirus by sequences within the long terminal repeat. In: Nature. 1984 ; Vol. 308, No. 5958. pp. 467-470.
@article{9f0071ab5184446281acfb3bef01811f,
title = "Determination of the leukaemogenicity of a murine retrovirus by sequences within the long terminal repeat",
abstract = "Although the murine retrovirus SL3-3 is highly leukaemo-genic 1,2, in both the structure of its genome and in its properties of replication in tissue culture it closely resembles the non-leukaemogenic retrovirus Akv (refs 3, 4). An.earlier investigation of the properties of recombinant SL3-3-Akv viruses localized the major determinant of leukaemogenicity outside the env gene, in a region of the viral genome that includes the gag gene and the noncoding long terminal repeat (LTR)4. To localize the determinant of SL3-3's leukaemogenicity more precisely we have now construced a recombinant provirus containing the LTR of SL3-3 and the coding region of Akv. The leukaemogenicity of these recombinants demonstrates that the determinant of leukaemogenicity ties within the SL3-3 LTR. Nucleotide sequencing of the LTRs of SL3-3 and Akv shows that they differ by a set of changes in the region thought to contain a transcriptional enhancer element. We suggest that enhancer region sequences are the major determinants of leukaemogenicity in these viruses.",
author = "Jack Lenz and Daniel Celander and Crowther, {Robert L.} and Roberto Patarca and Perkins, {Dennis W.} and Haseltine, {William A.}",
year = "1984",
doi = "10.1038/308467a0",
language = "English (US)",
volume = "308",
pages = "467--470",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "5958",

}

TY - JOUR

T1 - Determination of the leukaemogenicity of a murine retrovirus by sequences within the long terminal repeat

AU - Lenz, Jack

AU - Celander, Daniel

AU - Crowther, Robert L.

AU - Patarca, Roberto

AU - Perkins, Dennis W.

AU - Haseltine, William A.

PY - 1984

Y1 - 1984

N2 - Although the murine retrovirus SL3-3 is highly leukaemo-genic 1,2, in both the structure of its genome and in its properties of replication in tissue culture it closely resembles the non-leukaemogenic retrovirus Akv (refs 3, 4). An.earlier investigation of the properties of recombinant SL3-3-Akv viruses localized the major determinant of leukaemogenicity outside the env gene, in a region of the viral genome that includes the gag gene and the noncoding long terminal repeat (LTR)4. To localize the determinant of SL3-3's leukaemogenicity more precisely we have now construced a recombinant provirus containing the LTR of SL3-3 and the coding region of Akv. The leukaemogenicity of these recombinants demonstrates that the determinant of leukaemogenicity ties within the SL3-3 LTR. Nucleotide sequencing of the LTRs of SL3-3 and Akv shows that they differ by a set of changes in the region thought to contain a transcriptional enhancer element. We suggest that enhancer region sequences are the major determinants of leukaemogenicity in these viruses.

AB - Although the murine retrovirus SL3-3 is highly leukaemo-genic 1,2, in both the structure of its genome and in its properties of replication in tissue culture it closely resembles the non-leukaemogenic retrovirus Akv (refs 3, 4). An.earlier investigation of the properties of recombinant SL3-3-Akv viruses localized the major determinant of leukaemogenicity outside the env gene, in a region of the viral genome that includes the gag gene and the noncoding long terminal repeat (LTR)4. To localize the determinant of SL3-3's leukaemogenicity more precisely we have now construced a recombinant provirus containing the LTR of SL3-3 and the coding region of Akv. The leukaemogenicity of these recombinants demonstrates that the determinant of leukaemogenicity ties within the SL3-3 LTR. Nucleotide sequencing of the LTRs of SL3-3 and Akv shows that they differ by a set of changes in the region thought to contain a transcriptional enhancer element. We suggest that enhancer region sequences are the major determinants of leukaemogenicity in these viruses.

UR - http://www.scopus.com/inward/record.url?scp=0021270238&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021270238&partnerID=8YFLogxK

U2 - 10.1038/308467a0

DO - 10.1038/308467a0

M3 - Article

VL - 308

SP - 467

EP - 470

JO - Nature

JF - Nature

SN - 0028-0836

IS - 5958

ER -