TY - JOUR
T1 - Determination of the concentrations of oligosaccharides, complex type carbohydrates, and glycoproteins using the phenol-sulfuric acid method
AU - Saha, Anat K.
AU - Brewer, Curtis F.
N1 - Funding Information:
+ This work was supported by Grant CA-16054 from the National Cancer Institute, Department of Health, Education and Welfare, Core Grant P30 CA-13330 from the same agency (CFB). The NMR facility at AECOM was supported by Instrumentation Grant I-SlO-RR02309 from the National Institute of Health and DMB-8413723 from the National Science Foundation. * Corresponding author.
PY - 1994/2/17
Y1 - 1994/2/17
N2 - The concentrations of methyl glycosides, oligosaccharides, glycopeptides, and glycoproteins can be accurately determined by using calibration curves composed of the appropriate monosaccharide(s) obtained with a modified version of the colorimetric phenol-sulfuric acid method. Calibration curves of μg sugar vs. 490 nm for Man, Glc, or Gal are shown to provide reliable determinations (typically ±3-4%) of corresponding methyl glycosides and linear and branched-chain oligosaccharides containing the corresponding reactive hexose residue. For complex oligosaccharides containing a known mixture of reactive hexose units, the appropriate mixture of monosaccharides are shown to provide equally accurate calbration curves for concentration determinations. In the case of the soybean agglutinin, which is a tetramer possessing one Man9 oligomannose-type chain per subunit, the proein concentration was determined from the Man calibration curve which agreed with that obtained from the molar extinction coefficient of the protein.
AB - The concentrations of methyl glycosides, oligosaccharides, glycopeptides, and glycoproteins can be accurately determined by using calibration curves composed of the appropriate monosaccharide(s) obtained with a modified version of the colorimetric phenol-sulfuric acid method. Calibration curves of μg sugar vs. 490 nm for Man, Glc, or Gal are shown to provide reliable determinations (typically ±3-4%) of corresponding methyl glycosides and linear and branched-chain oligosaccharides containing the corresponding reactive hexose residue. For complex oligosaccharides containing a known mixture of reactive hexose units, the appropriate mixture of monosaccharides are shown to provide equally accurate calbration curves for concentration determinations. In the case of the soybean agglutinin, which is a tetramer possessing one Man9 oligomannose-type chain per subunit, the proein concentration was determined from the Man calibration curve which agreed with that obtained from the molar extinction coefficient of the protein.
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U2 - 10.1016/0008-6215(94)84249-3
DO - 10.1016/0008-6215(94)84249-3
M3 - Article
C2 - 8180982
AN - SCOPUS:0028380173
SN - 0008-6215
VL - 254
SP - 157
EP - 167
JO - Carbohydrate Research
JF - Carbohydrate Research
IS - C
ER -