Determination of calcium transport and phosphoprotein phosphatase activity in microsomes from respiratory and vascular smooth muscle

Howard Sands, James Mascali, Elisabeth M. Paietta

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

1. 1. Calcium transport into microsomal vesicles of respiratory (tracheal) smooth muscle was characterized. This calcium transport was ATP dependent and stimulated by the presence of the oxalate ion. The magnitude of transport was similar to that reported for microsomes from other types of smooth muscle. 2. 2. Bovine and rabbit, heavy and light microsomes were isolated from respiratory (tracheal) and vascualar (aortic) smooth muscle. Preincubation of these vesicles with cyclic AMP and protein kinase did not alter the transport of calcium into the vesicles. There was no evidence of phosphate incorporatio into microsomal membrane proteins. Similar results were obtained if phosphorylase b kinase replaced the combination of cyclic AMP and protein kinase during the preincubation. 3. 3. The phosphoprotein phosphatase activity of cardiac sarcoplasmic reticulum and smooth muscle microsomes was determined. The activity of this enzyme was found to be several-fold less in the cardiac sarcoplasmic reticulum than in various smooth muscle microsome preparations.

Original languageEnglish (US)
Pages (from-to)223-234
Number of pages12
JournalBBA - General Subjects
Volume500
Issue number2
DOIs
StatePublished - Dec 22 1977
Externally publishedYes

Fingerprint

Phosphoprotein Phosphatases
Microsomes
Vascular Smooth Muscle
Smooth Muscle
Muscle
Calcium
Adenylate Kinase
Sarcoplasmic Reticulum
Cyclic AMP
Protein Kinases
Phosphorylase Kinase
Oxalates
Membrane Proteins
Adenosine Triphosphate
Phosphates
Ions
Rabbits
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Medicine(all)

Cite this

Determination of calcium transport and phosphoprotein phosphatase activity in microsomes from respiratory and vascular smooth muscle. / Sands, Howard; Mascali, James; Paietta, Elisabeth M.

In: BBA - General Subjects, Vol. 500, No. 2, 22.12.1977, p. 223-234.

Research output: Contribution to journalArticle

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