Determination of acrylamide and glycidamide in rat plasma by reversed-phase high performance liquid chromatography

David S. Barber, Jason Hunt, Richard M. LoPachin, Marion Ehrich

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33 Scopus citations


Acrylamide is a widely used monomer that produces peripheral neuropathy. It is metabolized to the epoxide, glycidamide, which is also considered to be neurotoxic. A new reversed-phase high-performance liquid chromatography (HPLC) method is described that permits simultaneous determination of acrylamide and glycidamide in rat plasma. Samples were deproteinized with acetonitrile and chromatography was performed using isocratic elution and UV absorption detection. The limits of detection for acrylamide and glycidamide were 0.05 and 0.25 μg/ml in plasma, respectively, and recovery of both analytes was greater than 90%. The assay was linear from 0.1 to 100 μg/ml for acrylamide and from 0.5 to 100 μg/ml for glycidamide. Variation over the range of the standard curve was less than 15%. The method was used to determine the concentration - time profiles of acrylamide and glycidamide in the plasma of acrylamide-treated rats.

Original languageEnglish (US)
Pages (from-to)289-293
Number of pages5
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Issue number2
Publication statusPublished - Jul 15 2001



  • Acrylamide
  • Glycidamide

ASJC Scopus subject areas

  • Chemistry(all)

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