Determination of a glucose-dependent futile recycling rate constant from an intraperitoneal glucose tolerance test

Jun Xu, W. N Paul Lee, Gary Xiao, Chuck Trujillo, Vicky Chang, Lilia Blanco, Felicia Hernandez, Beau Chung, Sahar Makabi, Sayed Ahmed, Sara Bassilian, Mohammed Saad, Irwin J. Kurland

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Increased glucose cycling between glucose and glucose-6-phosphate is characteristic of insulin resistance and hyperglycemia seen with Type II diabetes. Traditionally, glucose cycling is determined by the difference between hepatic glucose output measured with separate [2-3H]glucose and [6-3H]glucose infusions. We demonstrate a novel method for determining hepatic glucose recycling from an intraperitoneal glucose tolerance test (IPGTT). A single tracer, [1, 2-13C2]glucose (a M2 glucose isotopomer), was administered at 1mg/g body weight to 4-month-old C57BL/6 mice. Hepatic glucose recycling was monitored by the appearance of a plasma M1 isotopomer of glucose, which is produced by the action of the pentose cycle on the M2 glucose isotopomer in the liver. The initial M2 enrichment was 56% and decreased to 13% at the end of 3h, and the M1 enrichment peaked at 2h. The ratio of plasma M1/M2 glucose increased linearly with time to ∼25%, and the regression of the M1/M2 ratio against time gives a slope, termed the in vivo glucose-dependent futile recycling rate constant kHR·kHR estimates glucose/glucose-6-phosphate futile cycling, along with glucose recycling through the pentose cycle. These observations demonstrate complex substrate cycling during an IPGTT using a single stable isotope tracer.

Original languageEnglish (US)
Pages (from-to)238-246
Number of pages9
JournalAnalytical Biochemistry
Volume315
Issue number2
DOIs
StatePublished - Apr 15 2003
Externally publishedYes

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Recycling
Glucose Tolerance Test
Rate constants
Glucose
Substrate Cycling
Pentoses
Glucose-6-Phosphate
Liver
Plasmas
Inbred C57BL Mouse
Isotopes
Hyperglycemia
Type 2 Diabetes Mellitus
Medical problems
Insulin Resistance

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Determination of a glucose-dependent futile recycling rate constant from an intraperitoneal glucose tolerance test. / Xu, Jun; Lee, W. N Paul; Xiao, Gary; Trujillo, Chuck; Chang, Vicky; Blanco, Lilia; Hernandez, Felicia; Chung, Beau; Makabi, Sahar; Ahmed, Sayed; Bassilian, Sara; Saad, Mohammed; Kurland, Irwin J.

In: Analytical Biochemistry, Vol. 315, No. 2, 15.04.2003, p. 238-246.

Research output: Contribution to journalArticle

Xu, J, Lee, WNP, Xiao, G, Trujillo, C, Chang, V, Blanco, L, Hernandez, F, Chung, B, Makabi, S, Ahmed, S, Bassilian, S, Saad, M & Kurland, IJ 2003, 'Determination of a glucose-dependent futile recycling rate constant from an intraperitoneal glucose tolerance test', Analytical Biochemistry, vol. 315, no. 2, pp. 238-246. https://doi.org/10.1016/S0003-2697(02)00709-1
Xu, Jun ; Lee, W. N Paul ; Xiao, Gary ; Trujillo, Chuck ; Chang, Vicky ; Blanco, Lilia ; Hernandez, Felicia ; Chung, Beau ; Makabi, Sahar ; Ahmed, Sayed ; Bassilian, Sara ; Saad, Mohammed ; Kurland, Irwin J. / Determination of a glucose-dependent futile recycling rate constant from an intraperitoneal glucose tolerance test. In: Analytical Biochemistry. 2003 ; Vol. 315, No. 2. pp. 238-246.
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