Determinants recognized by murine rheumatoid factors: Molecular localization using a panel of mouse myeloma variant immunoglobulins

V. Stassin, P. G. Coulie, B. K. Birshtein, D. S. Secher, J. Van Snick

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Abstract

The structures recognized by monoclonal anti-IgG1 rheumatoid factors (RF) were localized by testing their reactivity with mutant immunoglobulins carrying γ1 chains that lacked either the C(H)1 or the C(H)3 domains. While optimal binding was observed in the absence of C(H)1, deletion of C(H)3 completely abolished the reactivity of all but one of the 71 monoclonal RF tested. Similar experiments were carried out with IgG2a- and IgG2b-specific RF by using variant immunoglobulins carrying various hybrid γ2a-γ2b heavy chains. It was found that both the polyclonal RF produced by autoimmune strains, MRL/MpJ-1pr and NZB/BinJ, and most of the monoclonal RF derived from normal strains, BALB/c, C57Bl/6, and 129/Sv, were directed against determinants located in a segment spanning the C-terminal 8 residues of the C(H)2 domain and the complete C(H)3 domain.

Original languageEnglish (US)
Pages (from-to)1763-1768
Number of pages6
JournalJournal of Experimental Medicine
Volume158
Issue number5
StatePublished - 1983

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Rheumatoid Factor
Immunoglobulins
Immunoglobulin G
myeloma immunoglobulins

ASJC Scopus subject areas

  • Immunology

Cite this

Determinants recognized by murine rheumatoid factors : Molecular localization using a panel of mouse myeloma variant immunoglobulins. / Stassin, V.; Coulie, P. G.; Birshtein, B. K.; Secher, D. S.; Van Snick, J.

In: Journal of Experimental Medicine, Vol. 158, No. 5, 1983, p. 1763-1768.

Research output: Contribution to journalArticle

Stassin, V. ; Coulie, P. G. ; Birshtein, B. K. ; Secher, D. S. ; Van Snick, J. / Determinants recognized by murine rheumatoid factors : Molecular localization using a panel of mouse myeloma variant immunoglobulins. In: Journal of Experimental Medicine. 1983 ; Vol. 158, No. 5. pp. 1763-1768.
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