Determinants of leptin gene expression in fat depots of lean mice

Yiying Zhang, Kai Ying Guo, Patricia A. Diaz, Moonseong Heo, Rudolph L. Leibel

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

The relationship of leptin gene expression to adipocyte volume was investigated in lean 10-wk-old male C57BL/6J mice. mRNA levels for leptin, insulin receptor, glucocorticoid receptor, and tumor necrosis factor (TNF)-α in inguinal, epididymal, and retroperitoneal adipose tissues were quantified and related to adipocyte volume. Leptin mRNA levels were highly correlated with adipocyte volume within each fat depot. Multiple regression analysis of pooled data from the three depots showed that leptin mRNA levels were strongly correlated with adipocyte volumes (β = 0.84, P < 0.001) and, to a smaller degree, with glucocorticoid receptor mRNA levels (β = 0.36, P < 0.001). Depot of origin had no effect (P > 0.9). Rates of leptin secretion in vitro were strongly correlated with leptin mRNA levels (r = 0.89, P < 0.001). mRNA levels for TNF-α, insulin receptor, and glucocorticoid receptor showed no significant correlation with adipocyte volume. These results demonstrate that depot-specific differences in leptin gene expression are mainly related to the volumes of the constituent adipocytes. The strong correlation between leptin gene expression and adipocyte volume supports leptin's physiological role as a humoral signal of fat mass.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume282
Issue number1 51-1
StatePublished - 2002
Externally publishedYes

Fingerprint

Leptin
Adipocytes
Fats
Gene Expression
Messenger RNA
Insulin Receptor
Glucocorticoid Receptors
Leptin Receptors
Intra-Abdominal Fat
Groin
Tumor Necrosis Factor Receptors
Inbred C57BL Mouse
Tumor Necrosis Factor-alpha
Regression Analysis

Keywords

  • Glucocorticoid receptor
  • Insulin receptor
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Physiology

Cite this

Determinants of leptin gene expression in fat depots of lean mice. / Zhang, Yiying; Guo, Kai Ying; Diaz, Patricia A.; Heo, Moonseong; Leibel, Rudolph L.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 282, No. 1 51-1, 2002.

Research output: Contribution to journalArticle

Zhang, Yiying ; Guo, Kai Ying ; Diaz, Patricia A. ; Heo, Moonseong ; Leibel, Rudolph L. / Determinants of leptin gene expression in fat depots of lean mice. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 2002 ; Vol. 282, No. 1 51-1.
@article{0dc12958ec83460fa5a7c822eadb5fc8,
title = "Determinants of leptin gene expression in fat depots of lean mice",
abstract = "The relationship of leptin gene expression to adipocyte volume was investigated in lean 10-wk-old male C57BL/6J mice. mRNA levels for leptin, insulin receptor, glucocorticoid receptor, and tumor necrosis factor (TNF)-α in inguinal, epididymal, and retroperitoneal adipose tissues were quantified and related to adipocyte volume. Leptin mRNA levels were highly correlated with adipocyte volume within each fat depot. Multiple regression analysis of pooled data from the three depots showed that leptin mRNA levels were strongly correlated with adipocyte volumes (β = 0.84, P < 0.001) and, to a smaller degree, with glucocorticoid receptor mRNA levels (β = 0.36, P < 0.001). Depot of origin had no effect (P > 0.9). Rates of leptin secretion in vitro were strongly correlated with leptin mRNA levels (r = 0.89, P < 0.001). mRNA levels for TNF-α, insulin receptor, and glucocorticoid receptor showed no significant correlation with adipocyte volume. These results demonstrate that depot-specific differences in leptin gene expression are mainly related to the volumes of the constituent adipocytes. The strong correlation between leptin gene expression and adipocyte volume supports leptin's physiological role as a humoral signal of fat mass.",
keywords = "Glucocorticoid receptor, Insulin receptor, Tumor necrosis factor-α",
author = "Yiying Zhang and Guo, {Kai Ying} and Diaz, {Patricia A.} and Moonseong Heo and Leibel, {Rudolph L.}",
year = "2002",
language = "English (US)",
volume = "282",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "1 51-1",

}

TY - JOUR

T1 - Determinants of leptin gene expression in fat depots of lean mice

AU - Zhang, Yiying

AU - Guo, Kai Ying

AU - Diaz, Patricia A.

AU - Heo, Moonseong

AU - Leibel, Rudolph L.

PY - 2002

Y1 - 2002

N2 - The relationship of leptin gene expression to adipocyte volume was investigated in lean 10-wk-old male C57BL/6J mice. mRNA levels for leptin, insulin receptor, glucocorticoid receptor, and tumor necrosis factor (TNF)-α in inguinal, epididymal, and retroperitoneal adipose tissues were quantified and related to adipocyte volume. Leptin mRNA levels were highly correlated with adipocyte volume within each fat depot. Multiple regression analysis of pooled data from the three depots showed that leptin mRNA levels were strongly correlated with adipocyte volumes (β = 0.84, P < 0.001) and, to a smaller degree, with glucocorticoid receptor mRNA levels (β = 0.36, P < 0.001). Depot of origin had no effect (P > 0.9). Rates of leptin secretion in vitro were strongly correlated with leptin mRNA levels (r = 0.89, P < 0.001). mRNA levels for TNF-α, insulin receptor, and glucocorticoid receptor showed no significant correlation with adipocyte volume. These results demonstrate that depot-specific differences in leptin gene expression are mainly related to the volumes of the constituent adipocytes. The strong correlation between leptin gene expression and adipocyte volume supports leptin's physiological role as a humoral signal of fat mass.

AB - The relationship of leptin gene expression to adipocyte volume was investigated in lean 10-wk-old male C57BL/6J mice. mRNA levels for leptin, insulin receptor, glucocorticoid receptor, and tumor necrosis factor (TNF)-α in inguinal, epididymal, and retroperitoneal adipose tissues were quantified and related to adipocyte volume. Leptin mRNA levels were highly correlated with adipocyte volume within each fat depot. Multiple regression analysis of pooled data from the three depots showed that leptin mRNA levels were strongly correlated with adipocyte volumes (β = 0.84, P < 0.001) and, to a smaller degree, with glucocorticoid receptor mRNA levels (β = 0.36, P < 0.001). Depot of origin had no effect (P > 0.9). Rates of leptin secretion in vitro were strongly correlated with leptin mRNA levels (r = 0.89, P < 0.001). mRNA levels for TNF-α, insulin receptor, and glucocorticoid receptor showed no significant correlation with adipocyte volume. These results demonstrate that depot-specific differences in leptin gene expression are mainly related to the volumes of the constituent adipocytes. The strong correlation between leptin gene expression and adipocyte volume supports leptin's physiological role as a humoral signal of fat mass.

KW - Glucocorticoid receptor

KW - Insulin receptor

KW - Tumor necrosis factor-α

UR - http://www.scopus.com/inward/record.url?scp=0036084221&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036084221&partnerID=8YFLogxK

M3 - Article

C2 - 11742842

AN - SCOPUS:0036084221

VL - 282

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

IS - 1 51-1

ER -