Detection of the major pertussis toxin substrate of human leukocytes with antisera raised against synthetic peptides

Judith Falloon; Harry Malech; Graeme Milligan; Cecilia Unson; Richard Kahn; Paul Goldsmith; Allen Spiegel

doi:10.1016/0014-5793(86)81141-3

Detection of the major pertussis toxin substrate of human leukocytes with antisera raised against synthetic peptides

Judith Falloon, Harry Malech, Graeme Milligan, Cecilia Unson, Richard Kahn, Paul Goldsmith, Allen Spiegel

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Antisera raised against the carboxy-terminal decapeptide (KENLKDCGLF) of transducin-α detected the 40 kDa, major pertussis toxin substrate of human neutrophils. The antisera also detected this protein in undifferentiated HL-60 and U937 cells, and revealed an approx. 2-fold increase in protein/mg membrane protein with differentiation into mature phagocytic cells. The results provide direct immunochemical evidence for the presence of a novel, pertussis toxin-sensitive guanine nucleotide-binding protein in human leukocytes.

Original language	English (US)
Pages (from-to)	352-356
Number of pages	5
Journal	FEBS Letters
Volume	209
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(86)81141-3
State	Published - Dec 15 1986
Externally published	Yes

Keywords

GTP-binding protein
Phosphoinositide breakdown
Signal transduction

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(86)81141-3

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abstract = "Antisera raised against the carboxy-terminal decapeptide (KENLKDCGLF) of transducin-α detected the 40 kDa, major pertussis toxin substrate of human neutrophils. The antisera also detected this protein in undifferentiated HL-60 and U937 cells, and revealed an approx. 2-fold increase in protein/mg membrane protein with differentiation into mature phagocytic cells. The results provide direct immunochemical evidence for the presence of a novel, pertussis toxin-sensitive guanine nucleotide-binding protein in human leukocytes.",

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T1 - Detection of the major pertussis toxin substrate of human leukocytes with antisera raised against synthetic peptides

AU - Falloon, Judith

AU - Malech, Harry

AU - Milligan, Graeme

AU - Unson, Cecilia

AU - Kahn, Richard

AU - Goldsmith, Paul

AU - Spiegel, Allen

PY - 1986/12/15

Y1 - 1986/12/15

N2 - Antisera raised against the carboxy-terminal decapeptide (KENLKDCGLF) of transducin-α detected the 40 kDa, major pertussis toxin substrate of human neutrophils. The antisera also detected this protein in undifferentiated HL-60 and U937 cells, and revealed an approx. 2-fold increase in protein/mg membrane protein with differentiation into mature phagocytic cells. The results provide direct immunochemical evidence for the presence of a novel, pertussis toxin-sensitive guanine nucleotide-binding protein in human leukocytes.

AB - Antisera raised against the carboxy-terminal decapeptide (KENLKDCGLF) of transducin-α detected the 40 kDa, major pertussis toxin substrate of human neutrophils. The antisera also detected this protein in undifferentiated HL-60 and U937 cells, and revealed an approx. 2-fold increase in protein/mg membrane protein with differentiation into mature phagocytic cells. The results provide direct immunochemical evidence for the presence of a novel, pertussis toxin-sensitive guanine nucleotide-binding protein in human leukocytes.

KW - GTP-binding protein

KW - Phosphoinositide breakdown

KW - Signal transduction

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Detection of the major pertussis toxin substrate of human leukocytes with antisera raised against synthetic peptides

Abstract

Keywords

ASJC Scopus subject areas

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