Detection of the major pertussis toxin substrate of human leukocytes with antisera raised against synthetic peptides

Judith Falloon, Harry Malech, Graeme Milligan, Cecilia Unson, Richard Kahn, Paul Goldsmith, Allen Spiegel

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Antisera raised against the carboxy-terminal decapeptide (KENLKDCGLF) of transducin-α detected the 40 kDa, major pertussis toxin substrate of human neutrophils. The antisera also detected this protein in undifferentiated HL-60 and U937 cells, and revealed an approx. 2-fold increase in protein/mg membrane protein with differentiation into mature phagocytic cells. The results provide direct immunochemical evidence for the presence of a novel, pertussis toxin-sensitive guanine nucleotide-binding protein in human leukocytes.

Original languageEnglish (US)
Pages (from-to)352-356
Number of pages5
JournalFEBS Letters
Volume209
Issue number2
DOIs
StatePublished - Dec 15 1986
Externally publishedYes

Keywords

  • GTP-binding protein
  • Phosphoinositide breakdown
  • Signal transduction

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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