Detection of the major pertussis toxin substrate of human leukocytes with antisera raised against synthetic peptides

Judith Falloon, Harry Malech, Graeme Milligan, Cecilia Unson, Richard Kahn, Paul Goldsmith, Allen M. Spiegel

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Antisera raised against the carboxy-terminal decapeptide (KENLKDCGLF) of transducin-α detected the 40 kDa, major pertussis toxin substrate of human neutrophils. The antisera also detected this protein in undifferentiated HL-60 and U937 cells, and revealed an approx. 2-fold increase in protein/mg membrane protein with differentiation into mature phagocytic cells. The results provide direct immunochemical evidence for the presence of a novel, pertussis toxin-sensitive guanine nucleotide-binding protein in human leukocytes.

Original languageEnglish (US)
Pages (from-to)352-356
Number of pages5
JournalFEBS Letters
Volume209
Issue number2
DOIs
StatePublished - Dec 15 1986
Externally publishedYes

Fingerprint

Pertussis Toxin
Immune Sera
Leukocytes
Transducin
U937 Cells
Peptides
Guanine Nucleotides
HL-60 Cells
Substrates
Phagocytes
Carrier Proteins
Membrane Proteins
Proteins
Neutrophils

Keywords

  • GTP-binding protein
  • Phosphoinositide breakdown
  • Signal transduction

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Detection of the major pertussis toxin substrate of human leukocytes with antisera raised against synthetic peptides. / Falloon, Judith; Malech, Harry; Milligan, Graeme; Unson, Cecilia; Kahn, Richard; Goldsmith, Paul; Spiegel, Allen M.

In: FEBS Letters, Vol. 209, No. 2, 15.12.1986, p. 352-356.

Research output: Contribution to journalArticle

Falloon, Judith ; Malech, Harry ; Milligan, Graeme ; Unson, Cecilia ; Kahn, Richard ; Goldsmith, Paul ; Spiegel, Allen M. / Detection of the major pertussis toxin substrate of human leukocytes with antisera raised against synthetic peptides. In: FEBS Letters. 1986 ; Vol. 209, No. 2. pp. 352-356.
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