Detection of multiple forms of G in HL60 cells

Philip M. Murphy, Brock Eide, Paul Goldsmith, Mark Brann, Peter Gierschik, Allen M. Spiegel, Harry L. Malech

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Comparison of cDNA sequences from multiple sources predicts a genus of highly homologous but structurally distinct G protein α-subunits, designated as G, that may include the α-subunit of the functionally defined adenylate cyclase inhibitory G protein. Using specific oligonucleotide probes on Northern blots, we show that Giα-2 and Giα-3, but not Giα-1, are expressed in HL60 cells. Antisera raised against synthetic peptides from regions predicted to be conserved (AS6) and divergent (LE3) among G subtypes bind to a 40 kDa protein in Western blots of HL60 membranes. AS6 identifies an additional protein at 41 kDa. Thus, Northern blot and immunoblot results show that at least two G subtypes, a 40 kDa protein likely to correspond to Giα-2 and a 41 kDa protein possibly representing Giα-3, may be expressed in a single cell type.

Original languageEnglish (US)
Pages (from-to)81-86
Number of pages6
JournalFEBS Letters
Volume221
Issue number1
DOIs
StatePublished - Aug 31 1987
Externally publishedYes

Fingerprint

HL-60 Cells
GTP-Binding Proteins
Northern Blotting
Proteins
Oligonucleotide Probes
Protein Subunits
Adenylyl Cyclases
Immune Sera
Complementary DNA
Western Blotting
Membranes
Peptides

Keywords

  • (HL60 cell)
  • Differentiation
  • Guanine nucleotide regulatory protein
  • mRNA
  • Pertussis toxin
  • Signal transduction

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Murphy, P. M., Eide, B., Goldsmith, P., Brann, M., Gierschik, P., Spiegel, A. M., & Malech, H. L. (1987). Detection of multiple forms of G in HL60 cells. FEBS Letters, 221(1), 81-86. https://doi.org/10.1016/0014-5793(87)80356-3

Detection of multiple forms of G in HL60 cells. / Murphy, Philip M.; Eide, Brock; Goldsmith, Paul; Brann, Mark; Gierschik, Peter; Spiegel, Allen M.; Malech, Harry L.

In: FEBS Letters, Vol. 221, No. 1, 31.08.1987, p. 81-86.

Research output: Contribution to journalArticle

Murphy, PM, Eide, B, Goldsmith, P, Brann, M, Gierschik, P, Spiegel, AM & Malech, HL 1987, 'Detection of multiple forms of G in HL60 cells', FEBS Letters, vol. 221, no. 1, pp. 81-86. https://doi.org/10.1016/0014-5793(87)80356-3
Murphy PM, Eide B, Goldsmith P, Brann M, Gierschik P, Spiegel AM et al. Detection of multiple forms of G in HL60 cells. FEBS Letters. 1987 Aug 31;221(1):81-86. https://doi.org/10.1016/0014-5793(87)80356-3
Murphy, Philip M. ; Eide, Brock ; Goldsmith, Paul ; Brann, Mark ; Gierschik, Peter ; Spiegel, Allen M. ; Malech, Harry L. / Detection of multiple forms of G in HL60 cells. In: FEBS Letters. 1987 ; Vol. 221, No. 1. pp. 81-86.
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