Detection of multiple forms of G in HL60 cells

Philip M. Murphy, Brock Eide, Paul Goldsmith, Mark Brann, Peter Gierschik, Allen Spiegel, Harry L. Malech

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Comparison of cDNA sequences from multiple sources predicts a genus of highly homologous but structurally distinct G protein α-subunits, designated as G, that may include the α-subunit of the functionally defined adenylate cyclase inhibitory G protein. Using specific oligonucleotide probes on Northern blots, we show that Giα-2 and Giα-3, but not Giα-1, are expressed in HL60 cells. Antisera raised against synthetic peptides from regions predicted to be conserved (AS6) and divergent (LE3) among G subtypes bind to a 40 kDa protein in Western blots of HL60 membranes. AS6 identifies an additional protein at 41 kDa. Thus, Northern blot and immunoblot results show that at least two G subtypes, a 40 kDa protein likely to correspond to Giα-2 and a 41 kDa protein possibly representing Giα-3, may be expressed in a single cell type.

Original languageEnglish (US)
Pages (from-to)81-86
Number of pages6
JournalFEBS Letters
Volume221
Issue number1
DOIs
StatePublished - Aug 31 1987
Externally publishedYes

Keywords

  • (HL60 cell)
  • Differentiation
  • Guanine nucleotide regulatory protein
  • Pertussis toxin
  • Signal transduction
  • mRNA

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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    Murphy, P. M., Eide, B., Goldsmith, P., Brann, M., Gierschik, P., Spiegel, A., & Malech, H. L. (1987). Detection of multiple forms of G in HL60 cells. FEBS Letters, 221(1), 81-86. https://doi.org/10.1016/0014-5793(87)80356-3