Abstract
Comparison of cDNA sequences from multiple sources predicts a genus of highly homologous but structurally distinct G protein α-subunits, designated as Giα, that may include the α-subunit of the functionally defined adenylate cyclase inhibitory G protein. Using specific oligonucleotide probes on Northern blots, we show that Giα-2 and Giα-3, but not Giα-1, are expressed in HL60 cells. Antisera raised against synthetic peptides from regions predicted to be conserved (AS6) and divergent (LE3) among Giα subtypes bind to a 40 kDa protein in Western blots of HL60 membranes. AS6 identifies an additional protein at 41 kDa. Thus, Northern blot and immunoblot results show that at least two Giα subtypes, a 40 kDa protein likely to correspond to Giα-2 and a 41 kDa protein possibly representing Giα-3, may be expressed in a single cell type.
Original language | English (US) |
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Pages (from-to) | 81-86 |
Number of pages | 6 |
Journal | FEBS Letters |
Volume | 221 |
Issue number | 1 |
DOIs | |
State | Published - Aug 31 1987 |
Externally published | Yes |
Keywords
- (HL60 cell)
- Differentiation
- Guanine nucleotide regulatory protein
- Pertussis toxin
- Signal transduction
- mRNA
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology