Detection of human papillomavirus DNA in cytologically normal women and subsequent cervical squamous intraepithelial lesions

Kai Li Liaw, Andrew G. Glass, M. Michele Manos, Catherine E. Greer, David R. Scott, Mark Sherman, Robert D. Burk, Robert J. Kurman, Sholom Wacholder, Brenda B. Rush, Diane M. Cadell, Patti Lawler, David Tabor, Mark Schiffman

Research output: Contribution to journalArticle

211 Citations (Scopus)

Abstract

Background: Human papillomavirus (HPV) infection has been strongly associated with cervical carcinoma and its cytologic precursors, squamous intraepithelial lesions (SIL). We investigated the risk of SIL prospectively following polymerase chain reaction (PCR)-based DNA testing for a wide range of genital HPV types in a cohort of initially cytologically normal women, to clarify the role of HPV in the etiology of SIL. Methods: starting in April 1989, 17 654 women who were receiving routine cytologic screening at Kaiser Permanente (Portland, OR) were followed for the development of incident SIL. During follow-up, 380 incident case patients and 1037 matched control subjects were eligible for this nested case-control study. Cervical lavages collected at enrollment and, later, at the time of case diagnosis (or the corresponding time for selection of control subjects) were tested for HPV DNA using a PCR-based method. The data were analyzed as contingency tables with two-sided P values or, for multivariable analyses, using odds ratios (ORs) with 95% confidence intervals (CIs). Results: In comparison with initially HPV-negative women, women who tested positive for HPV DNA at enrollment were 3.8 times (95% CI = 2.6-5.5) more likely to have low-grade SIL subsequently diagnosed for the first time during follow-up and 12.7 times more likely (95% CI = 6.2-25.9) to develop high-grade SIL. At the time of diagnosis, the cross-sectional association of HPV DNA and SIL was extremely strong (OR = 44.4 and 95% CI = 24.2-81.5 for low-grade SIL and OR = 67.1 and 95% CI 19.3- 233.7 for high-grade SIL). HPV16 was the virus type most predictive of SIL, even low-grade SIL. Conclusions: These findings are consistent with the hypothesis that HPV infection is the primary cause of cervical neoplasia. Furthermore, they support HPV vaccine research to prevent cervical cancer and efforts to develop HPV DNA diagnostic tests.

Original languageEnglish (US)
Pages (from-to)954-960
Number of pages7
JournalJournal of the National Cancer Institute
Volume91
Issue number11
StatePublished - Jun 2 1999

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DNA
Confidence Intervals
Odds Ratio
Papillomavirus Infections
Human Papillomavirus DNA Tests
Squamous Intraepithelial Lesions of the Cervix
Papillomavirus Vaccines
Polymerase Chain Reaction
Therapeutic Irrigation
Routine Diagnostic Tests
Uterine Cervical Neoplasms
Patient Selection
Case-Control Studies
Viruses
Carcinoma
Research
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Liaw, K. L., Glass, A. G., Manos, M. M., Greer, C. E., Scott, D. R., Sherman, M., ... Schiffman, M. (1999). Detection of human papillomavirus DNA in cytologically normal women and subsequent cervical squamous intraepithelial lesions. Journal of the National Cancer Institute, 91(11), 954-960.

Detection of human papillomavirus DNA in cytologically normal women and subsequent cervical squamous intraepithelial lesions. / Liaw, Kai Li; Glass, Andrew G.; Manos, M. Michele; Greer, Catherine E.; Scott, David R.; Sherman, Mark; Burk, Robert D.; Kurman, Robert J.; Wacholder, Sholom; Rush, Brenda B.; Cadell, Diane M.; Lawler, Patti; Tabor, David; Schiffman, Mark.

In: Journal of the National Cancer Institute, Vol. 91, No. 11, 02.06.1999, p. 954-960.

Research output: Contribution to journalArticle

Liaw, KL, Glass, AG, Manos, MM, Greer, CE, Scott, DR, Sherman, M, Burk, RD, Kurman, RJ, Wacholder, S, Rush, BB, Cadell, DM, Lawler, P, Tabor, D & Schiffman, M 1999, 'Detection of human papillomavirus DNA in cytologically normal women and subsequent cervical squamous intraepithelial lesions', Journal of the National Cancer Institute, vol. 91, no. 11, pp. 954-960.
Liaw, Kai Li ; Glass, Andrew G. ; Manos, M. Michele ; Greer, Catherine E. ; Scott, David R. ; Sherman, Mark ; Burk, Robert D. ; Kurman, Robert J. ; Wacholder, Sholom ; Rush, Brenda B. ; Cadell, Diane M. ; Lawler, Patti ; Tabor, David ; Schiffman, Mark. / Detection of human papillomavirus DNA in cytologically normal women and subsequent cervical squamous intraepithelial lesions. In: Journal of the National Cancer Institute. 1999 ; Vol. 91, No. 11. pp. 954-960.
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abstract = "Background: Human papillomavirus (HPV) infection has been strongly associated with cervical carcinoma and its cytologic precursors, squamous intraepithelial lesions (SIL). We investigated the risk of SIL prospectively following polymerase chain reaction (PCR)-based DNA testing for a wide range of genital HPV types in a cohort of initially cytologically normal women, to clarify the role of HPV in the etiology of SIL. Methods: starting in April 1989, 17 654 women who were receiving routine cytologic screening at Kaiser Permanente (Portland, OR) were followed for the development of incident SIL. During follow-up, 380 incident case patients and 1037 matched control subjects were eligible for this nested case-control study. Cervical lavages collected at enrollment and, later, at the time of case diagnosis (or the corresponding time for selection of control subjects) were tested for HPV DNA using a PCR-based method. The data were analyzed as contingency tables with two-sided P values or, for multivariable analyses, using odds ratios (ORs) with 95{\%} confidence intervals (CIs). Results: In comparison with initially HPV-negative women, women who tested positive for HPV DNA at enrollment were 3.8 times (95{\%} CI = 2.6-5.5) more likely to have low-grade SIL subsequently diagnosed for the first time during follow-up and 12.7 times more likely (95{\%} CI = 6.2-25.9) to develop high-grade SIL. At the time of diagnosis, the cross-sectional association of HPV DNA and SIL was extremely strong (OR = 44.4 and 95{\%} CI = 24.2-81.5 for low-grade SIL and OR = 67.1 and 95{\%} CI 19.3- 233.7 for high-grade SIL). HPV16 was the virus type most predictive of SIL, even low-grade SIL. Conclusions: These findings are consistent with the hypothesis that HPV infection is the primary cause of cervical neoplasia. Furthermore, they support HPV vaccine research to prevent cervical cancer and efforts to develop HPV DNA diagnostic tests.",
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AU - Greer, Catherine E.

AU - Scott, David R.

AU - Sherman, Mark

AU - Burk, Robert D.

AU - Kurman, Robert J.

AU - Wacholder, Sholom

AU - Rush, Brenda B.

AU - Cadell, Diane M.

AU - Lawler, Patti

AU - Tabor, David

AU - Schiffman, Mark

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