Detection of hepatitis C virus RNA sequences in B-cell non-Hodgkin lymphoma

Sebastian J. Karavattathayyil, Gururaj Kalkeri, Huifeng Jenny Liu, Paul Gaglio, Robert F. Garry, John R. Krause, Srikanta Dash

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Serologic testing shows that hepatitis C virus (HCV) may have a role in the pathogenesis of B-cell non-Hodgkin lymphomas (B-cell NHLs). We tried to demonstrate HCV RNA sequences in paraffin-embedded tissue from B-cell NHLs by reverse-transcription double polymerase chain reaction (RT-PCR) and Southern blotting. We studied 31 consecutive cases of B-cell NHLs; lymph nodes from 32 patients with diseases other than B-cell NHL were negative controls. Positive-strand HCV RNA was tested with primers for the 5' untranslated region. Replicative negative strand HCV RNA was tested with strand-specific RT-PCR for the 5' untranslated region. Immunohistochemical staining for HCV was done using an antibody to HCV core protein. Positive-strand HCV RNA was detected in 8 patients with B-cell NHL; negative-strand HCV RNA was detected in 6 of these cases, indicating viral replication. All control cases were negative for HCV RNA. Immunohistochemistry showed no staining of lymphoma cells for HCV core proteins in any case. HCV and B-cell NHLs may be associated. RT-PCR on paraffin-embedded lymphoma tissue is an alternative method of testing for HCV. The value of immunohistochemistry could not be ascertained. The exact role of HCV in the pathogenesis of B-cell NHL needs to be studied further.

Original languageEnglish (US)
Pages (from-to)391-398
Number of pages8
JournalAmerican journal of clinical pathology
Volume113
Issue number3
DOIs
StatePublished - 2000

Keywords

  • Hepatitis C
  • Non-Hodgkin lymphoma
  • Paraffin section
  • RT-PCR
  • Reverse-transcription double polymerase chain reaction

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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