Detection of chemical mutagens using muta mouse

A transgenic mouse model

A. J W Hoorn, L. L. Custer, B. C. Myhr, D. Brusick, J. Gossen, Jan Vijg

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

A transgenic mouse strain with a high copy number of rescuable lac Z sequences was evaluated for its effectiveness in detecting lacZ- mutations in selected tissues. Procarbazine, cyclophosphamide, ethylnitrosourea, 7, 12-dimethylbenz [a] anthracene (DMBA), acrylamide and chlorambucil were tested following either single or repeated dosing regimens. Bone marrow, liver, skin and testis tissues were selected to assess as target sites for mutation. Bone marrow, liver and testis tissues were examined for mutation following exposures to ethylnitrosourea and chlorambucil. Increased mutant frequencies were found for both chemicals in all three tissues. Bone marrow tissue was examined for mutation following procarbazine, cyclophosphamide and acrylamide exposures, and skin was examined for mutation following dermal application of DMBA. Mutation induction was observed in all cases. The results btained from this investigation demonstrate the applicability of this transgenic mouse as an effective model to detect and analyze gene mutation in selected organs including germinal tissues. Studies of organotrophic chemical mutagens and carcinogens are possible with this model as are studies of the susceptibility of germinal tissues to mutagen exposures.

Original languageEnglish (US)
Pages (from-to)7-10
Number of pages4
JournalMutagenesis
Volume8
Issue number1
DOIs
StatePublished - Jan 1993
Externally publishedYes

Fingerprint

Mutagens
Transgenic Mice
Mouse
Mutation
Tissue
Bone
Ethylnitrosourea
Procarbazine
Chlorambucil
9,10-Dimethyl-1,2-benzanthracene
Acrylamide
Bone Marrow
Liver
Cyclophosphamide
Skin
Testis
Model
Carcinogens
Anthracene
Mutant

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Hoorn, A. J. W., Custer, L. L., Myhr, B. C., Brusick, D., Gossen, J., & Vijg, J. (1993). Detection of chemical mutagens using muta mouse: A transgenic mouse model. Mutagenesis, 8(1), 7-10. https://doi.org/10.1093/mutage/8.1.7

Detection of chemical mutagens using muta mouse : A transgenic mouse model. / Hoorn, A. J W; Custer, L. L.; Myhr, B. C.; Brusick, D.; Gossen, J.; Vijg, Jan.

In: Mutagenesis, Vol. 8, No. 1, 01.1993, p. 7-10.

Research output: Contribution to journalArticle

Hoorn, AJW, Custer, LL, Myhr, BC, Brusick, D, Gossen, J & Vijg, J 1993, 'Detection of chemical mutagens using muta mouse: A transgenic mouse model', Mutagenesis, vol. 8, no. 1, pp. 7-10. https://doi.org/10.1093/mutage/8.1.7
Hoorn, A. J W ; Custer, L. L. ; Myhr, B. C. ; Brusick, D. ; Gossen, J. ; Vijg, Jan. / Detection of chemical mutagens using muta mouse : A transgenic mouse model. In: Mutagenesis. 1993 ; Vol. 8, No. 1. pp. 7-10.
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