Detection of bladder cancer recurrence by microsatellite analysis of urine

Gabriel Steiner, Mark P. Schoenberg, Jurgen F. Linn, L. I. Mao, David Sidransky

Research output: Contribution to journalArticle

235 Citations (Scopus)

Abstract

A reliable, noninvasive method for monitoring patients with transitional cell carcinoma (TCC) of the bladder would be of great clinical benefit. Cystoscopy is currently the 'gold standard,' but it is invasive, expensive and uncomfortable for the patient. Recently, we demonstrated a novel approach for the detection of primary bladder cancer based on microsatellite analysis of urine DNA. To determine the feasibility of this technique for following- up patients with TCC, we tested serial urine samples from 21 patients who had been treated for bladder cancer with 20 polymorphic microsatellite markers in a blinded fashion. We detected recurrent lesions in 10 out of 11 patients and correctly predicted the existence of a neoplastic cell population in the urine of two patients, 4 and 6 months before cystoscopic evidence of the tumor. The assay was negative in 10 of 10 patients who had no evident cancer. Microsatellite analysis of urine sediment represents a novel and potentially powerful clinical tool for the detection of recurrent bladder cancer.

Original languageEnglish (US)
Pages (from-to)621-624
Number of pages4
JournalNature Medicine
Volume3
Issue number6
DOIs
StatePublished - 1997
Externally publishedYes

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Urinary Bladder Neoplasms
Microsatellite Repeats
Urine
Recurrence
Cells
Patient monitoring
Transitional Cell Carcinoma
Tumors
Assays
Sediments
Cystoscopy
DNA
Physiologic Monitoring
Neoplasms
Urinary Bladder
Population

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Detection of bladder cancer recurrence by microsatellite analysis of urine. / Steiner, Gabriel; Schoenberg, Mark P.; Linn, Jurgen F.; Mao, L. I.; Sidransky, David.

In: Nature Medicine, Vol. 3, No. 6, 1997, p. 621-624.

Research output: Contribution to journalArticle

Steiner, Gabriel ; Schoenberg, Mark P. ; Linn, Jurgen F. ; Mao, L. I. ; Sidransky, David. / Detection of bladder cancer recurrence by microsatellite analysis of urine. In: Nature Medicine. 1997 ; Vol. 3, No. 6. pp. 621-624.
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