Detection of antibodies to small nuclear ribonucleoproteins and small cytoplasmic ribonucleoproteins using unlabeled cell extracts

M. S. Forman, M. Nakamura, T. Mimori, C. Gelpi, J. A. Hardin

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

RNA molecules immunoprecipitated with sera from patients who have rheumatic diseases can be readily detected in polyacrylamide gels by using ethidium bromide and silver stains. With these stains, we found that RNA patterns characteristic of a broad range of specific small nuclear ribonucleoproteins and small cytoplasmic ribonucleoproteins were recognizable. The stains correctly identified antibodies to ribonucleoproteins in 33 (92%) of 36 patient sera selected for study because of known antibody specificities. The silver stain method detected antibodies to ribonucleoproteins in 25 (76%) of 33 patients with classic systemic lupus erythematosus, a frequency that approximated the frequency observed in the Lerner-Steitz assay, which is based on autoradiography. This approach considerably simplifies the latter radioimmunoassay with minimal loss of precision and sensitivity.

Original languageEnglish (US)
Pages (from-to)1356-1361
Number of pages6
JournalArthritis and Rheumatism
Volume28
Issue number12
DOIs
StatePublished - 1985
Externally publishedYes

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Small Cytoplasmic Ribonucleoproteins
Small Nuclear Ribonucleoproteins
Cell Extracts
Coloring Agents
Ribonucleoproteins
Antibodies
Silver
RNA
Ethidium
Antibody Specificity
Rheumatic Diseases
Autoradiography
Serum
Systemic Lupus Erythematosus
Radioimmunoassay

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Detection of antibodies to small nuclear ribonucleoproteins and small cytoplasmic ribonucleoproteins using unlabeled cell extracts. / Forman, M. S.; Nakamura, M.; Mimori, T.; Gelpi, C.; Hardin, J. A.

In: Arthritis and Rheumatism, Vol. 28, No. 12, 1985, p. 1356-1361.

Research output: Contribution to journalArticle

Forman, M. S. ; Nakamura, M. ; Mimori, T. ; Gelpi, C. ; Hardin, J. A. / Detection of antibodies to small nuclear ribonucleoproteins and small cytoplasmic ribonucleoproteins using unlabeled cell extracts. In: Arthritis and Rheumatism. 1985 ; Vol. 28, No. 12. pp. 1356-1361.
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